Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12 Pt 1
|
| pubmed:dateCreated |
1999-3-3
|
| pubmed:abstractText |
CTL lines have now been generated against defined peptides of a range of human tumor-associated antigens (TAAs). One of the potential uses of these epitope-specific CTLs is in adoptive transfer immunotherapy. This is a modality, however, that will require long-term in vitro culture of CTLs. To date, little has been reported concerning the phenotypic stability of human epitope-specific CTLs as a consequence of long-term in vitro propagation via peptide stimulation. We report here the serial phenotypic characterization of a CTL line directed against an immunodominant epitope (YLSGANLNL, designated CAP-1) of human carcinoembryonic antigen (CEA). This CTL line was derived from peripheral blood mononuclear cells of a patient with metastatic carcinoma who had been treated with a recombinant CEA-vaccinia vaccine in a Phase I trial; the CTLs were analyzed through 20 in vitro cycle passages of stimulation with CAP-1 peptide and interleukin 2 in the presence of autologous antigen-presenting cells. The CTL line was shown to be phenotypically stable in terms of high levels of cytokine (IFN-gamma, tumor necrosis factor, and granulocyte-macrophage colony-stimulating factor) production, expression of homing-adhesion molecules, ability to lyse peptide-pulsed targets, and ability to lyse human carcinoma cells endogenously expressing CEA in a MHC-restricted manner. Vbeta T-cell receptor gene usage was also analyzed. These studies thus present a rationale for the use of long-term cultured epitope-specific human CTLs, directed against a human TAA for potential adoptive transfer immunotherapy protocols.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD58,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinoembryonic Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alpha4,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1078-0432
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
3
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2439-49
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9815645-Antigens, CD,
pubmed-meshheading:9815645-Antigens, CD4,
pubmed-meshheading:9815645-Antigens, CD58,
pubmed-meshheading:9815645-Antigens, CD8,
pubmed-meshheading:9815645-Carcinoembryonic Antigen,
pubmed-meshheading:9815645-Cell Line,
pubmed-meshheading:9815645-Colonic Neoplasms,
pubmed-meshheading:9815645-Colorectal Neoplasms,
pubmed-meshheading:9815645-Cytotoxicity, Immunologic,
pubmed-meshheading:9815645-Epitopes,
pubmed-meshheading:9815645-Flow Cytometry,
pubmed-meshheading:9815645-Humans,
pubmed-meshheading:9815645-Immunophenotyping,
pubmed-meshheading:9815645-Integrin alpha4,
pubmed-meshheading:9815645-Intercellular Adhesion Molecule-1,
pubmed-meshheading:9815645-Polymerase Chain Reaction,
pubmed-meshheading:9815645-Recombinant Proteins,
pubmed-meshheading:9815645-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9815645-Transfection,
pubmed-meshheading:9815645-Tumor Cells, Cultured
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Phenotypic stability of a cytotoxic T-cell line directed against an immunodominant epitope of human carcinoembryonic antigen.
|
| pubmed:affiliation |
Laboratory of Tumor Immunology and Biology, National Cancer Institute, NIH, Bethesda, Maryland 20892-1750, USA.
|
| pubmed:publicationType |
Journal Article
|