Linked Life Data

9814441

Source:http://linkedlifedata.com/resource/pubmed/id/9814441

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-11-25
pubmed:abstractText
Formaldehyde (FA) is a genotoxic substance, induces tumors in the nasal epithelium of rats, and is suspected to be a human carcinogen. As a primary DNA lesion, FA induces DNA-protein crosslinks (DPC) and the formation of DPC has been used as a measure of exposure for risk estimation. However, the significance of DPC for mutagenesis and carcinogenesis is at present poorly understood. We therefore performed comparative investigations on the induction of DPC and other genetic endpoints by FA in V79 Chinese hamster cells. The amount of DPC was comparatively determined with the K-SDS assay and the comet assay. Both tests gave similar results but the comet assay was much foster and easier to perform. Our results show that FA significantly induces DPC, sister-chromatid exchanges, and micronuclei in the same range of concentrations, parallel to the induction of cytotoxicity (relative cloning efficiency). In contrast, treatment of V79 cells with FA did not induce gene mutations in the HPRT test even after variations of the treatment protocol. Our results indicate that FA-induced DPC seem to be related to cytotoxicity and clastogenicity but do not lead to the formation of gene mutations in mammalian cells. It is suggested that FA-induced DPC do not cause gene mutations that are involved in FA-induced carcinogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0893-6692
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
260-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Significance of formaldehyde-induced DNA-protein crosslinks for mutagenesis.
pubmed:affiliation
Universitätsklinikum Ulm, Abteilung Medizinische Genetik, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't