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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1999-2-4
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pubmed:abstractText |
Importin alpha is the nuclear localization signal (NLS) receptor that is involved in the nuclear import of proteins containing basic NLSs. Using importin alpha as a tool, we were interested in determining whether the cytoskeleton could function in the transport of NLS-containing proteins from the cytoplasm to the nucleus. Double-labeling immunofluorescence studies showed that most of the cytoplasmic importin alpha coaligned with microtubules and microfilaments in tobacco protoplasts. Treatment of tobacco protoplasts with microtubule- or microfilament-depolymerizing agents disrupted the strands of importin alpha in the cytoplasm, whereas a microtubule-stabilizing agent had no effect. Biochemical analysis showed that importin alpha associated with microtubules and microfilaments in vitro in an NLS-dependent manner. The interaction of importin alpha with the cytoskeleton could be an essential element of protein transport from the cytoplasm to the nucleus in vivo.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-1332794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-1377237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-1533967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-1854481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-7706018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-7907279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8001131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8148877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8205004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8400874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8531796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8533089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8636214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8636215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8689555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8718621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8757785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8776900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8886989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8930133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-8953247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9013679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9060466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9159081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9193081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9214382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9218799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9261175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9312057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9323123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9330872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9351834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9362492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9418045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9438838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9438839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9490720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9506951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9671230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811789-9695836
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1040-4651
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1791-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:9811789-Actin Cytoskeleton,
pubmed-meshheading:9811789-Amino Acid Sequence,
pubmed-meshheading:9811789-Biological Transport, Active,
pubmed-meshheading:9811789-Cell Nucleus,
pubmed-meshheading:9811789-Cytoskeleton,
pubmed-meshheading:9811789-Karyopherins,
pubmed-meshheading:9811789-Microtubules,
pubmed-meshheading:9811789-Molecular Sequence Data,
pubmed-meshheading:9811789-Nuclear Localization Signals,
pubmed-meshheading:9811789-Nuclear Proteins,
pubmed-meshheading:9811789-Plant Proteins,
pubmed-meshheading:9811789-Plants, Toxic,
pubmed-meshheading:9811789-Protoplasts,
pubmed-meshheading:9811789-Tobacco,
pubmed-meshheading:9811789-alpha Karyopherins
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pubmed:year |
1998
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pubmed:articleTitle |
Nuclear localization signal receptor importin alpha associates with the cytoskeleton.
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pubmed:affiliation |
Department of Energy-Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1312, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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