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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1998-12-14
pubmed:abstractText
Zinc is an essential nutrient for all cells, but remarkably little is known regarding bacterial zinc transport and its regulation. We have identified three of the key components acting to maintain zinc homeostasis in Bacillus subtilis. Zur is a metalloregulatory protein related to the ferric uptake repressor (Fur) family of regulators and is required for the zinc-specific repression of two operons implicated in zinc uptake, yciC and ycdHIyceA. A zur mutant overexpresses the 45-kDa YciC membrane protein, and purified Zur binds specifically, and in a zinc-responsive manner, to an operator site overlapping the yciC control region. A similar operator precedes the ycdH-containing operon, which encodes an ABC transporter. Two lines of evidence suggest that the ycdH operon encodes a high-affinity zinc transporter whereas YciC may function as part of a lower-affinity pathway. First, a ycdH mutant is impaired in growth in low-zinc medium, and this growth defect is exacerbated by the additional presence of a yciC mutation. Second, mutation of ycdH, but not yciC, alters the regulation of both the yciC and ycdH operons such that much higher levels of exogenous zinc are required for repression. We conclude that Zur is a Fur-like repressor that controls the expression of two zinc homeostasis operons in response to zinc. Thus, Fur-like regulators control zinc homeostasis in addition to their previously characterized roles in regulating iron homeostasis, acid tolerance responses, and oxidative stress functions.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-1345911, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-1579110, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-1655697, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-1868094, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-1946347, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-2013568, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-2199796, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-2437099, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-2963952, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-2993806, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-7630724, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-7667267, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-7704253, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-7753029, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-8108390, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-8335620, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-8396117, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-8637895, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-8764488, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-8798516, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-8871549, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-8969508, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9254694, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9263657, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9271382, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9360976, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9364914, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9379902, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9384377, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9405611, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9466913, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9485406, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9680209, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9696746, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9701813, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9724772, http://linkedlifedata.com/resource/pubmed/commentcorrection/9811636-9765793
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
180
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5815-21
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:9811636-ATP-Binding Cassette Transporters, pubmed-meshheading:9811636-Amino Acid Sequence, pubmed-meshheading:9811636-Bacillus subtilis, pubmed-meshheading:9811636-Bacterial Proteins, pubmed-meshheading:9811636-Base Sequence, pubmed-meshheading:9811636-Biological Transport, pubmed-meshheading:9811636-DNA, Bacterial, pubmed-meshheading:9811636-DNA-Binding Proteins, pubmed-meshheading:9811636-Escherichia coli Proteins, pubmed-meshheading:9811636-Gene Expression Regulation, Bacterial, pubmed-meshheading:9811636-Membrane Proteins, pubmed-meshheading:9811636-Molecular Sequence Data, pubmed-meshheading:9811636-Mutagenesis, pubmed-meshheading:9811636-Operon, pubmed-meshheading:9811636-Promoter Regions, Genetic, pubmed-meshheading:9811636-Repressor Proteins, pubmed-meshheading:9811636-Sequence Homology, Amino Acid, pubmed-meshheading:9811636-Transcription, Genetic, pubmed-meshheading:9811636-Zinc
pubmed:year
1998
pubmed:articleTitle
Identification of a zinc-specific metalloregulatory protein, Zur, controlling zinc transport operons in Bacillus subtilis.
pubmed:affiliation
Section of Microbiology, Wing Hall, Cornell University, Ithaca, NY 14853-8101, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.