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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
24
|
| pubmed:dateCreated |
1999-2-16
|
| pubmed:databankReference | |
| pubmed:abstractText |
We have identified Xenopus Brain Factor 2 (XBF-2) as a potent neuralizing activity in an expression cloning screen. In ectodermal explants, XBF-2 converts cells from an epidermal to a neural fate. Such explants contain neurons with distinct axonal profiles and express both anterior and posterior central nervous system (CNS) markers. In striking contrast to X-ngnR-1a or X-NeuroD, ectopic expression of XBF-2 in Xenopus embryos results in an expansion of the neural plate to the ventral midline. The enlarged neural plate consists predominantly of undifferentiated neurons. XBF-2 lies downstream of the BMP antagonists noggin, cerberus, and gremlin since ectodermal explants expressing these molecules exhibit strong expression of XBF-2. While XBF-2 does not upregulate the expression of secreted neural inducers, it downregulates the transcription of BMP-4, an epidermal inducer. We show that XBF-2 acts as a transcriptional repressor and that its effects can be phenocopied with either the engrailed or hairy repressor domain fused to the XBF-2 DNA-binding domain. A fusion of the DNA-binding domain to the activator domain of VP16 blocks the effects of XBF-2 and prevents neural plate development in the embryo. This provides evidence that a transcriptional repressor can affect both regional neural development and neurogenesis in vertebrates.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0950-1991
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
125
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
5019-31
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9811586-Amino Acid Sequence,
pubmed-meshheading:9811586-Animals,
pubmed-meshheading:9811586-Bone Morphogenetic Proteins,
pubmed-meshheading:9811586-COS Cells,
pubmed-meshheading:9811586-Cell Differentiation,
pubmed-meshheading:9811586-Central Nervous System,
pubmed-meshheading:9811586-DNA-Binding Proteins,
pubmed-meshheading:9811586-Ectoderm,
pubmed-meshheading:9811586-Gene Expression Regulation, Developmental,
pubmed-meshheading:9811586-Immunohistochemistry,
pubmed-meshheading:9811586-In Situ Hybridization,
pubmed-meshheading:9811586-Microinjections,
pubmed-meshheading:9811586-Molecular Sequence Data,
pubmed-meshheading:9811586-Nerve Tissue Proteins,
pubmed-meshheading:9811586-RNA, Messenger,
pubmed-meshheading:9811586-Repressor Proteins,
pubmed-meshheading:9811586-Sequence Alignment,
pubmed-meshheading:9811586-Transcription Factors,
pubmed-meshheading:9811586-Transfection,
pubmed-meshheading:9811586-Xenopus
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
XBF-2 is a transcriptional repressor that converts ectoderm into neural tissue.
|
| pubmed:affiliation |
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|