Linked Life Data

9809678

Source:http://linkedlifedata.com/resource/pubmed/id/9809678

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1999-1-7
pubmed:abstractText
The Qa-2 antigen, the product of the Ped (Preimplantation embryo development) gene, is a glycosylphosphatidylinositol-linked cell surface protein encoded in the Q region of the mouse major histocompatibility complex (MHC). Ped fast (Qa-2+) mouse strains have significantly higher preimplantation embryo cleavage rates both in vivo and in vitro than Ped slow (Qa-2-) mice. In this study, we determined whether the Ped fast phenotype of blastocysts is due to an increased number of blastomeres in the trophectoderm (TE), the inner cell mass (ICM), or both. We also analysed the Ped gene expression pattern, both at the mRNA and at the protein level, in these lineages. Blastocysts were collected from the congenic mouse strains B6.K2 (Qa-2 +) and B6.K1 (Qa-2-). We performed reverse transcription-polymerase chain reaction (PCR) and Immuno-PCR and found that the Ped gene is expressed at the mRNA and protein level in whole embryos and in isolated ICM cells. Lastly, we differentially stained embryos from these strains and found that B6.K2 blastocysts had significantly higher cell numbers (P < 0.05) in both the ICM and in the TE than B6.K1 blastocysts. These results suggest that Qa-2 expression in both the TE and the ICM of blastocysts directly contributes to the Ped phenotype.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1360-9947
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
966-71
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
The expression pattern of the Qa-2 antigen in mouse preimplantation embryos and its correlation with the Ped gene phenotype.
pubmed:affiliation
Department of Biology, Northeastern University, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.