Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1998-12-21
|
| pubmed:abstractText |
Clonal expansion of B-cell precursor acute lymphoblastic leukemia (ALL) is potentially regulated by survival, growth, and death signals transduced by the bone marrow (BM) microenvironment. Using a human BM stromal cell culture that supports the growth of normal human B-cell precursors, we established a pre-B ALL cell line designated BLIN-2. BLIN-2 has a clonal rearrangement of the Ig heavy chain locus, a dic(9;20) chromosomal abnormality, and a bi-allelic deletion of the p16(INK4a) and p19(ARF) genes. The most interesting feature of BLIN-2 is an absolute dependence on adherent human BM stromal cells for sustained survival and growth. BLIN-2 cultured in the absence of BM stromal cells undergo apoptosis, and direct contact with viable BM stromal cells is essential for optimal growth. BLIN-2 cells also grow on vascular cell adhesion molecule-1 (VCAM-1)-negative human skin fibroblasts, making it unlikely that a very late antigen-4 (VLA-4)/VCAM-1 interaction is required for BLIN-2 growth. Western blot analysis of BLIN-2 cells cultured in the presence or absence of BM stromal cells demonstrates that contact of BLIN-2 with BM stromal cells induces hyperphosphorylation of Rb. In contrast, the pre-B ALL cell line BLIN-1, which has a bi-allelic deletion of p16(INK4a) p19(ARF) but does not require BM stromal cells for growth, does not undergo Rb phosphorylation after BM stromal cell contact. The BLIN-2 cell line will facilitate identification of ligand/receptor interactions at the B-cell precursor/BM stromal cell interface and may provide new insight into microenvironmental regulation of leukemic cell survival and growth.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
92
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3817-28
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9808575-Apoptosis,
pubmed-meshheading:9808575-Bone Marrow Cells,
pubmed-meshheading:9808575-Cell Communication,
pubmed-meshheading:9808575-Cell Cycle,
pubmed-meshheading:9808575-Cells, Cultured,
pubmed-meshheading:9808575-Chromosomes, Human, Pair 9,
pubmed-meshheading:9808575-Coculture Techniques,
pubmed-meshheading:9808575-Female,
pubmed-meshheading:9808575-Fibroblasts,
pubmed-meshheading:9808575-Genes, p16,
pubmed-meshheading:9808575-Humans,
pubmed-meshheading:9808575-In Situ Hybridization, Fluorescence,
pubmed-meshheading:9808575-Infant,
pubmed-meshheading:9808575-Male,
pubmed-meshheading:9808575-Phosphorylation,
pubmed-meshheading:9808575-Polymerase Chain Reaction,
pubmed-meshheading:9808575-Precursor B-Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:9808575-Protein Processing, Post-Translational,
pubmed-meshheading:9808575-Retinoblastoma Protein,
pubmed-meshheading:9808575-Stromal Cells,
pubmed-meshheading:9808575-Tumor Cells, Cultured,
pubmed-meshheading:9808575-Vascular Cell Adhesion Molecule-1
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Development of a model for evaluating the interaction between human pre-B acute lymphoblastic leukemic cells and the bone marrow stromal cell microenvironment.
|
| pubmed:affiliation |
Department of Laboratory Medicine/Pathology, Center for Immunology, and the Cancer Center, University of Minnesota, Minneapolis, MN, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|