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pubmed-article:9808478pubmed:abstractTextWe have found that feeding Brown Norway (BN) rat spleen cells to Lewis rats prior to transplanting BN kidneys prolongs allograft survival (mean: 8.8 days in unfed rats, 21 days in the BN cell-fed rats; longest survival: 11 days without allo-feeding vs. 37 days with feeding). We have also found that feeding BN cells both before and after transplantation further extends survival (mean: 38 days; longest survival: 105 days). We also examined the cells infiltrating the grafts during the early stages of the allograft response (day 5). Using flow cytometry, we found a significant decrease in the number of leukocytes infiltrating the transplanted kidneys of fed animals. This decrease was mainly due to a drop in the number of infiltrating T cells. We also found that cytokine mRNA production by the graft-infiltrating lymphocytes, assessed by reverse transcription polymerase chain reaction, showed a significant increase in interleukin-4 and transforming-growth factor-beta mRNA in the graft-infiltrating lymphocytes of fed animals compared with the controls.lld:pubmed
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pubmed-article:9808478pubmed:articleTitleProlongation of survival of primary renal allografts by feeding of donor spleen cells.lld:pubmed
pubmed-article:9808478pubmed:affiliationDepartment of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada.lld:pubmed
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pubmed-article:9808478pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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