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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1998-11-25
|
| pubmed:abstractText |
We have found that feeding Brown Norway (BN) rat spleen cells to Lewis rats prior to transplanting BN kidneys prolongs allograft survival (mean: 8.8 days in unfed rats, 21 days in the BN cell-fed rats; longest survival: 11 days without allo-feeding vs. 37 days with feeding). We have also found that feeding BN cells both before and after transplantation further extends survival (mean: 38 days; longest survival: 105 days). We also examined the cells infiltrating the grafts during the early stages of the allograft response (day 5). Using flow cytometry, we found a significant decrease in the number of leukocytes infiltrating the transplanted kidneys of fed animals. This decrease was mainly due to a drop in the number of infiltrating T cells. We also found that cytokine mRNA production by the graft-infiltrating lymphocytes, assessed by reverse transcription polymerase chain reaction, showed a significant increase in interleukin-4 and transforming-growth factor-beta mRNA in the graft-infiltrating lymphocytes of fed animals compared with the controls.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0041-1337
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
27
|
| pubmed:volume |
66
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
976-82
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9808478-Administration, Oral,
pubmed-meshheading:9808478-Animals,
pubmed-meshheading:9808478-Cytokines,
pubmed-meshheading:9808478-Graft Survival,
pubmed-meshheading:9808478-Injections, Intravenous,
pubmed-meshheading:9808478-Intubation, Gastrointestinal,
pubmed-meshheading:9808478-Kidney,
pubmed-meshheading:9808478-Kidney Transplantation,
pubmed-meshheading:9808478-Leukocytes,
pubmed-meshheading:9808478-Male,
pubmed-meshheading:9808478-Portal Vein,
pubmed-meshheading:9808478-Postoperative Care,
pubmed-meshheading:9808478-Preoperative Care,
pubmed-meshheading:9808478-Rats,
pubmed-meshheading:9808478-Rats, Inbred BN,
pubmed-meshheading:9808478-Rats, Inbred Lew,
pubmed-meshheading:9808478-Spleen,
pubmed-meshheading:9808478-Time Factors,
pubmed-meshheading:9808478-Tissue Donors,
pubmed-meshheading:9808478-Transplantation, Homologous
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Prolongation of survival of primary renal allografts by feeding of donor spleen cells.
|
| pubmed:affiliation |
Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|