Linked Life Data

9807662

Source:http://linkedlifedata.com/resource/pubmed/id/9807662

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1999-1-22
pubmed:abstractText
1. The aims of the present study were to detect changes in superoxide anion (O2.-), nitric oxide (NO) and other reactive oxygen species (ROS) directly by measurement of chemiluminescence (CL) and to investigate the role of L-arginine, a nitric oxide synthase (NOS) substrate, and NG-nitro-L-arginine methyl ester (L-NAME), a NOS inhibitor, together with their molecular enantiomers D-arginine and D-NAME, in a rat mesenteric ischaemia-reperfusion (I/R) model. 2. Seventy-nine female Wistar albino rats were divided into eight groups. The first three groups underwent sham operation; group 1 was the control group, group 2 received L-arginine and group 3 received L-NAME. Ischaemia was produced in the remaining five groups by ligation of the superior mesenteric artery for 30 min followed by 60 min reperfusion. Group 4 rats were control I/R rats and groups 5-8 received either L-arginine, L-NAME, D-arginine or D-NAME, respectively. 3. Both luminol and lucigenin CL was significantly increased in I/R groups compared with sham-operated groups. L-Arginine significantly reduced CL measurements. D-Arginine was also protective, but not as much as L-arginine. Both L- and D-arginine had in vitro O2.- (-)scavenging potential, as tested by the xanthine-xanthine oxidase system. NG-Nitro-L-arginine methyl ester decreased lipid peroxidation values in addition to reducing CL measurements. Nitric oxide concentrations were significantly increased in I/R groups in comparison with sham-operated groups. Peroxynitrite formation was increased by I/R. Treatment with L-NAME was beneficial by reducing NO concentrations in the reperfused ileum. 4. In our I/R model, O2.-, NO and other ROS were increased. Although NOS inhibitors were effective in reducing oxidative damage, increasing NO concentrations with L-arginine was also beneficial, presumably due to the ability of L-arginine to inhibit phagocyte adherence and its radical scavenging potential. In fact, NO may have different effects in terms of tissue injury or protection depending on the concentration of oxygen and the haemodynamic state of the tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0305-1870
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
908-12
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:9807662-Acridines, pubmed-meshheading:9807662-Animals, pubmed-meshheading:9807662-Arginine, pubmed-meshheading:9807662-Enzyme Inhibitors, pubmed-meshheading:9807662-Female, pubmed-meshheading:9807662-Indicators and Reagents, pubmed-meshheading:9807662-Ischemia, pubmed-meshheading:9807662-Luminescent Measurements, pubmed-meshheading:9807662-Luminol, pubmed-meshheading:9807662-Mesenteric Artery, Superior, pubmed-meshheading:9807662-Mesentery, pubmed-meshheading:9807662-NG-Nitroarginine Methyl Ester, pubmed-meshheading:9807662-Nitric Oxide, pubmed-meshheading:9807662-Nitric Oxide Synthase, pubmed-meshheading:9807662-Rats, pubmed-meshheading:9807662-Rats, Wistar, pubmed-meshheading:9807662-Reactive Oxygen Species, pubmed-meshheading:9807662-Reperfusion, pubmed-meshheading:9807662-Spectrophotometry, pubmed-meshheading:9807662-Stereoisomerism, pubmed-meshheading:9807662-Superoxides
pubmed:year
1998
pubmed:articleTitle
Oxygen radicals and nitric oxide in rat mesenteric ischaemia-reperfusion: modulation by L-arginine and NG-nitro-L-arginine methyl ester.
pubmed:affiliation
Department of Biochemistry, School of Medicine, Marmara University, Istanbul, Turkey.
pubmed:publicationType
Journal Article