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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1999-1-6
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pubmed:abstractText |
Egr-1 (early-growth response factor-1) is a sequence-specific transcription factor that plays a regulatory role in the expression of many genes important for cell growth, development and the pathogenesis of disease. The transcriptional co-activators CBP (cAMP-response-element-binding-protein-binding protein) and p300 interact with sequence-specific transcription factors as well as components of the basal transcription machinery to facilitate RNA polymerase II recruitment and transcriptional initiation. Here we demonstrate a unique way in which Egr-1 physically and functionally interacts with CBP/p300 to modulate gene transcription. CBP/p300 potentiated Egr-1 mediated expression of 5-lipoxygenase (5-LO) promoter-reporter constructs, and the degree of trans-activation was proportional to the number of Egr-1 consensus binding sites present in wild-type and naturally occurring mutants of the 5-LO promoter. The N- and C-terminal domains of CBP interact with the transcriptional activation domain of Egr-1, as demonstrated by a mammalian two-hybrid assay. Direct protein-protein interactions between CBP/p300 and Egr-1 were demonstrated by glutathione S-transferase fusion-protein binding and co-immunoprecipitation/Western-blot studies. These data suggest that CBP and p300 act as transcriptional co-activators for Egr-1-mediated gene expression and that variations between individuals in such co-activation could serve as a genetic basis for variability in gene expression.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-181786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-2251250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-2820055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-3127059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-3672127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-7499234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-7523245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-7588605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-7593235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-7624335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-7673240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-7731805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-7754034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-7913207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-7918637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8028671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8413673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8502484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8596917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8598284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8616895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8621548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8666932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8668170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8702507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8703054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8779707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8848048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8917528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8918891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-8945521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-9045878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-9053449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-9062372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-9096323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-9194565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-9321833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9806899-9698605
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
336 ( Pt 1)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
183-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9806899-Animals,
pubmed-meshheading:9806899-COS Cells,
pubmed-meshheading:9806899-DNA-Binding Proteins,
pubmed-meshheading:9806899-Nuclear Proteins,
pubmed-meshheading:9806899-Precipitin Tests,
pubmed-meshheading:9806899-Protein Binding,
pubmed-meshheading:9806899-Recombinant Fusion Proteins,
pubmed-meshheading:9806899-Trans-Activators,
pubmed-meshheading:9806899-Transcription, Genetic,
pubmed-meshheading:9806899-Transcription Factors
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pubmed:year |
1998
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pubmed:articleTitle |
cAMP-response-element-binding-protein-binding protein (CBP) and p300 are transcriptional co-activators of early growth response factor-1 (Egr-1).
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pubmed:affiliation |
Vascular Research Division, Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, 221 Longwood Avenue, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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