Linked Life Data

9805351

Source:http://linkedlifedata.com/resource/pubmed/id/9805351

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-1-7
pubmed:abstractText
With the objective to investigate 3-deoxyglucosone (3-DG) mediated lysine crosslinks in vivo, we have isolated a lysine-3-DG-lysine crosslink from in vitro reaction of 3-DG and N-proportional to-acetyllysine (NAL). This crosslink, named as furopyrrolopyridine crosslink (FPPC), has intense blue fluorescence with absorption maxima at 235, 270 and 370 nm and emission maximum at 470 nm. The absorption and fluorescence spectra of FPPC were not altered in pHs ranging from 2-12, but the characteristic spectrum of FPPC (at pH 7.0) disappeared when it was reduced with sodium borohydride. FAB-MS showed that FPPC has a molecular mass of 611, equivalent to the reaction of two molecules each of NAL and 3-DG with the concomitant loss of 5 molecules of water. NMR data showed that FPPC has a pyridinium ring and four free hydroxy groups. Since acid hydrolyzed FPPC can be detected by amino acid analysis, we have determined its levels in the acid hydrolyzates of proteins glycated by 3-DG or in the acid hydrolyzates of normal aged, cataractous, diabetic and brunescent human lens proteins as well as in the acid hydrolyzed glycated hemoglobin, A0.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1082-6068
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
319-38
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Characterization of a blue fluorophore isolated from in vitro reaction of N-proportional to-acetyllysine and 3-deoxyglucosone.
pubmed:affiliation
Department of Ophthalmology, School of Medicine, University of Missouri, Columbia 65212, USA.
pubmed:publicationType
Journal Article