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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1999-6-2
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pubmed:abstractText |
Activation of primary resting T cells requires costimulation which can be delivered by the B7 molecules (CD80 and CD86) expressed on activated antigen-presenting cells (APC). In the present study, we examined in vitro effects of immunotoxins (ITs) composed of gelonin conjugated to mAbs against CD80 or CD86 (alphaCD80-IT and alphaCD86-IT). The specificity of both ITs was demonstrated using CD80 and CD86 transfected cell lines. In primary mixed lymphocyte cultures (MLCs), it was found that the average inhibitory capacity of alphaCD86-IT (72%) and alphaCD80-IT (30%) was significantly higher than alphaCD86 (54%) and alphaCD80 (11%). In reculture MLC experiments it was found that peripheral blood mononuclear cells pretreated with alphaCD86/alphaCD80 regained full stimulatory capacity whereas alphaCD86-IT/alphaCD80-IT pretreatment induced >95% loss of stimulatory capacity. Our results therefore demonstrate that these alphaB7-ITs functionally block B7-CD28 costimulatory signaling and eliminate activated APC.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/CD86 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/GEL protein, Gelonium multiflorum,
http://linkedlifedata.com/resource/pubmed/chemical/Immunotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Ribosome Inactivating Proteins...
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0001-2815
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
52
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
270-4
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9802607-Antigen-Antibody Reactions,
pubmed-meshheading:9802607-Antigen-Presenting Cells,
pubmed-meshheading:9802607-Antigens, CD,
pubmed-meshheading:9802607-Antigens, CD80,
pubmed-meshheading:9802607-Antigens, CD86,
pubmed-meshheading:9802607-Cells, Cultured,
pubmed-meshheading:9802607-Deoxyribonucleases,
pubmed-meshheading:9802607-Humans,
pubmed-meshheading:9802607-Immunity, Cellular,
pubmed-meshheading:9802607-Immunotoxins,
pubmed-meshheading:9802607-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:9802607-Membrane Glycoproteins,
pubmed-meshheading:9802607-Plant Proteins,
pubmed-meshheading:9802607-Protein Synthesis Inhibitors,
pubmed-meshheading:9802607-Ribosome Inactivating Proteins, Type 1,
pubmed-meshheading:9802607-T-Lymphocytes
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pubmed:year |
1998
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pubmed:articleTitle |
The effect of immunotoxins directed against CD80 and CD86 on primary T-cell alloresponses.
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pubmed:affiliation |
Department of Medical Immunology, Jordan Laboratory, University Hospital Utrecht, The Netherlands. h.g.otten@lab.azu.nl
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pubmed:publicationType |
Journal Article,
Comparative Study
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