Linked Life Data

9800648

Source:http://linkedlifedata.com/resource/pubmed/id/9800648

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1999-1-13
pubmed:abstractText
The simultaneous detection of the phase I metabolite of coumarin, 7-hydroxycoumarin, and the two phase II metabolites, 7-hydroxycoumarin-glucuronide and 7-hydroxycoumarin-sulphate by capillary electrophoresis with UV detection using liver slice incubations was investigated. Separation was carried out on an untreated fused silica capillary with detection at 320 nm. Separation was achieved in under 6 min with a total run time of 8 min. Both phase two metabolites were produced following an in vitro incubation of liver slices in Krebs-Hanseleit buffer with 100 microM 7-hydroxycoumarin. Limits of detection were 5.52 microM (2 micrograms ml-1) for the glucuronide, 2.21 microM (0.5 microgram ml-1) for the sulphate and 6.17 microM (1 microgram ml)-1 for 7-hydroxycoumarin. Mean inter- and intra-assay results are presented for all three analytes, respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1279-84
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Determination of 7-hydroxycoumarin and its glucuronide and sulphate conjugates in liver slice incubates by capillary zone electrophoresis.
pubmed:affiliation
School of Biological Sciences, Dublin City University, Glasnevin, Ireland.
pubmed:publicationType
Journal Article