|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
11
|
|
pubmed:dateCreated |
1998-12-4
|
|
pubmed:abstractText |
An analytical methodology was developed to quantitate the intracellular nucleotides including mono-, di-, and triphosphates and the diphosphocholine derivative of (-)-2', 3'-deoxy-3'-thiacytidine (3TC) in human peripheral blood mononuclear cells (PBMCs). The procedure includes the resolution of 3TC nucleotides by solid-phase extraction (SPE) on an anion-exchange cartridge, with subsequent enzyme digestion of the resulting phosphates to the parent drug that is ultimately quantitated by high-performance liquid chromatography with UV detection (HPLC-UV). Validation was performed with PBMCs from healthy donors exposed to [3H]3TC, leading to the formation of intracellular nucleotides that were quantitated by anion-exchange HPLC with radioactive detection (HPLC-RA). These nucleotide levels served as reference values and were used for cross-validation with data obtained by HPLC-UV. An excellent correlation was established between the results obtained by HPLC-RA and those obtained by HPLC-UV, with a slope of the regression lines close to unity and intercepts near nullity as well as a correlation coefficient close to unity for all 3TC phosphates. The assay was characterized by a limit of quantitation below 1 ng (amount on column) with a precision (percentage of coefficient of variation of repeated measurement) ranging from 0.8 to 18.1% and an accuracy (deviation of the amount determined by HPLC-UV from the nominal reference value) varying from -14.8 to 19.4%. This methodology was successfully applied to determine the quantity of 3TC nucleotides in PBMCs of a patient infected with human immunodeficiency virus after oral administration of 3TC and stavudine.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-1320365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-1321132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-1333199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-1347442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-1489190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-1590690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-1742082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-1776679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-1894938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-2159302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-2502840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-7511360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-7533197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-7685907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-7702641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-7860738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-8387298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-8387546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-8425021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-8633817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-8656507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-8673921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-8815701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-8913483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9797238-9098665
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0066-4804
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
42
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2989-95
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:9797238-Anti-HIV Agents,
pubmed-meshheading:9797238-Cells, Cultured,
pubmed-meshheading:9797238-Chromatography, High Pressure Liquid,
pubmed-meshheading:9797238-HIV Infections,
pubmed-meshheading:9797238-Humans,
pubmed-meshheading:9797238-Lamivudine,
pubmed-meshheading:9797238-Leukocytes, Mononuclear,
pubmed-meshheading:9797238-Nucleotides
|
|
pubmed:year |
1998
|
|
pubmed:articleTitle |
Intracellular nucleotides of (-)-2',3'-deoxy-3'-thiacytidine in peripheral blood mononuclear cells of a patient infected with human immunodeficiency virus.
|
|
pubmed:affiliation |
Department of Clinical Pharmacology, The Liver Center, Center for AIDS Research, University of Alabama at Birmingham, Birmingham, Alabama 35294-0019, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
