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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1998-11-5
|
| pubmed:databankReference | |
| pubmed:abstractText |
A Schistosoma mansoni cercarial cDNA expression library, constructed in lambda gt11, was screened using the IgG fraction of sera taken from rabbits vaccinated with irradiated cercariae. A positive cDNA clone (1,431 base pairs) was selected and characterized. The amino acid sequence predicted from the cDNA sequence identified a polypeptide of 363 amino acids that showed significant homology to different family members of the enzyme fructose-1,6-bisphosphate aldolase (EC 1.4.2.13). The identity was 66% and 65% with human C and A isoenzymes, respectively. Active sites and substrate-binding determinant analysis suggest that the isolated enzyme in terms of function resembles type A aldolase. The recombinant protein expressed in the vector pGEX-2T was found to be active enzymatically. Antibodies raised against the purified recombinant protein recognized a 40-kDa band in extracts from cercariae, schistosomula (5 and 25 days), adult worms, and eggs. Using immunocytochemistry, aldolase localized to the tegumental region of the adult worms.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0022-3395
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
84
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
954-60
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9794637-Amino Acid Sequence,
pubmed-meshheading:9794637-Animals,
pubmed-meshheading:9794637-Base Sequence,
pubmed-meshheading:9794637-Blotting, Western,
pubmed-meshheading:9794637-Cloning, Molecular,
pubmed-meshheading:9794637-Conserved Sequence,
pubmed-meshheading:9794637-DNA, Complementary,
pubmed-meshheading:9794637-DNA, Helminth,
pubmed-meshheading:9794637-Drosophila melanogaster,
pubmed-meshheading:9794637-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9794637-Female,
pubmed-meshheading:9794637-Fructose-Bisphosphate Aldolase,
pubmed-meshheading:9794637-Humans,
pubmed-meshheading:9794637-Immunohistochemistry,
pubmed-meshheading:9794637-Isoenzymes,
pubmed-meshheading:9794637-Molecular Sequence Data,
pubmed-meshheading:9794637-Rabbits,
pubmed-meshheading:9794637-Schistosoma mansoni,
pubmed-meshheading:9794637-Sequence Alignment
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Cloning and characterization of Schistosoma mansoni fructose-1,6-bisphosphate aldolase isoenzyme.
|
| pubmed:affiliation |
Theodore Bilharz Research Institute, Cairo, Egypt.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|