Linked Life Data

9794446

Source:http://linkedlifedata.com/resource/pubmed/id/9794446

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1998-11-18
pubmed:abstractText
Metachromatic cells in the peripheral blood of patients with asthma, allergy, or an allergic drug reaction were evaluated for their nuclear morphology, surface expression of the mast cell (MC) marker c-kit, surface expression of the basophil marker Bsp-1, and granule expression of MC proteases. Consistent with previous findings by others, Bsp-1+/metachromatic cells represented <1% of the cells in the peripheral blood of normal individuals. These cells generally contained segmented nuclei. Very little, if any, tryptase (Try), chymase (Chy), or carboxypeptidase A (CPA) was found in their granules, and very little, if any, c-kit was observed on their surfaces. The number of metachromatic cells increased in the peripheral blood of the three groups of patients. Like the basophils in normal individuals, most of these metachromatic cells contained segmented nuclei and expressed Bsp-1. However, in contrast to the basophils in normal individuals, many of the metachromatic cells in the three patient groups expressed c-kit, Try, Chy, and/or CPA. That the metachromatic cells in the blood of our patients have some features of MCs and some features of basophils suggests that human basophils and MCs are derived from a common progenitor. As assessed by the chloroacetate esterase cytochemical assay, the immunoreactive Chy in the peripheral blood of these patients is enzymatically active. Because MC proteases regulate numerous immunologic and other biologic systems, the expression of Try, Chy, and/or CPA in a peripheral blood-localized cell in an individual having asthma, allergy, or an allergic drug reaction has important clinical implications.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
161
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5079-86
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9794446-Acute Disease, pubmed-meshheading:9794446-Adult, pubmed-meshheading:9794446-Asthma, pubmed-meshheading:9794446-Basophils, pubmed-meshheading:9794446-Biological Markers, pubmed-meshheading:9794446-Carboxypeptidases, pubmed-meshheading:9794446-Carboxypeptidases A, pubmed-meshheading:9794446-Chymases, pubmed-meshheading:9794446-Convalescence, pubmed-meshheading:9794446-Drug Hypersensitivity, pubmed-meshheading:9794446-Enzyme Induction, pubmed-meshheading:9794446-Female, pubmed-meshheading:9794446-Humans, pubmed-meshheading:9794446-Hypersensitivity, Immediate, pubmed-meshheading:9794446-In Situ Hybridization, pubmed-meshheading:9794446-Isoenzymes, pubmed-meshheading:9794446-Leukocyte Count, pubmed-meshheading:9794446-Male, pubmed-meshheading:9794446-Mast Cells, pubmed-meshheading:9794446-Middle Aged, pubmed-meshheading:9794446-Serine Endopeptidases, pubmed-meshheading:9794446-Staining and Labeling, pubmed-meshheading:9794446-Tryptases
pubmed:year
1998
pubmed:articleTitle
Identification of basophilic cells that express mast cell granule proteases in the peripheral blood of asthma, allergy, and drug-reactive patients.
pubmed:affiliation
Department of Medicine, University of New South Wales, Australia.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't