Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1998-11-18
|
| pubmed:databankReference | |
| pubmed:abstractText |
Mammalian complement components factor B and C2 act as proteolytic subunits of the C3 convertases in the alternative and the classical activation pathways, respectively, and are believed to have diverged from a common ancestor by gene duplication. However, it is unclear when the B/C2 duplication occurred. Here, we describe two diverged B/C2-like cDNA clones (B/C2-A and B/C2-B) isolated from a bony fish, the common carp (Cyprinus carpio). B/C2-A shares the same domain structure as the factor B and C2 complement components of vertebrates reported so far and shows a close similarity to zebrafish B and medaka fish B/C2. These teleost sequences show almost the same degree of similarity to C2 and B of higher vertebrates. In contrast, B/C2-B has a novel structural feature in that it contains four short consensus repeat modules and does not have a close relative upon phylogenetic analysis. Northern blotting revealed the presence of two transcripts with different sizes for both the B/C2-A and B/C2-B in the hepatopancreas of the carp. Southern blotting suggested the presence of multiple genes for B/C2-A and a single gene for B/C2-B. Although structural features of B/C2-B are slightly more C2-like than B-like, B/C2-B has a crucial amino acid substitution in the serine protease domain, which makes it unlikely that B/C2-B functions as a C3 convertase. A possible phylogenetic relationship between the two carp sequences and mammalian C2 and B is discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
161
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4811-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9794413-Amino Acid Sequence,
pubmed-meshheading:9794413-Animals,
pubmed-meshheading:9794413-Carps,
pubmed-meshheading:9794413-Complement C2,
pubmed-meshheading:9794413-Complement Factor B,
pubmed-meshheading:9794413-Consensus Sequence,
pubmed-meshheading:9794413-DNA, Complementary,
pubmed-meshheading:9794413-Evolution, Molecular,
pubmed-meshheading:9794413-Gene Duplication,
pubmed-meshheading:9794413-Genes,
pubmed-meshheading:9794413-Humans,
pubmed-meshheading:9794413-Mammals,
pubmed-meshheading:9794413-Molecular Probe Techniques,
pubmed-meshheading:9794413-Molecular Sequence Data,
pubmed-meshheading:9794413-Phylogeny,
pubmed-meshheading:9794413-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:9794413-Sequence Alignment,
pubmed-meshheading:9794413-Sequence Homology, Amino Acid,
pubmed-meshheading:9794413-Species Specificity
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Two diverged complement factor B/C2-like cDNA sequences from a teleost, the common carp (Cyprinus carpio).
|
| pubmed:affiliation |
Laboratory of Marine Biochemistry, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|