Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1998-11-12
|
| pubmed:abstractText |
We studied PTEN/MMAC1, a newly discovered candidate tumor suppressor gene at 10q23.3, for mutations in lung cancer. One hundred and thirty-six lung cancer cell line DNAs (66 small cell lung cancers, SCLC, 61 non-small cell lung cancers, NSCLC, four mesotheliomas, five extrapulmonary small cell cancers) were analysed for PTEN/MMAC1 homozygous deletions and five (8%) SCLC lines showed homozygous deletions interrupting the PTEN/MMAC1 gene. Using single stranded conformation polymorphism (SSCP) analysis, we screened the PTEN/MMAC1 open reading frame of 53 lung cancer cell line cDNAs for point mutations and found that 3/35 SCLCs and 3/18 NSCLCs contained homozygous amino acid sequence altering mutations. Northern blot analysis revealed that expression of the PTEN/MMAC1 gene was considerably lower in all the tumor cell lines with point mutations while no expression was detected for cell lines with PTEN/MMAC1 homozygous deletions. Mutation analysis of 22 uncultured, microdissected, primary SCLC tumors and metastases showed two silent mutations, and two apparent homozygous deletions. We also discovered a processed pseudogene (PTEN2) which has 98.5% nt identity to PTEN/MMAC1, that needs to be accounted for in cDNA mutation analysis. Our findings suggest that genetic abnormalities of the PTEN/MMAC1 gene are only involved in a relatively small subset of lung cancers.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/PTEN Phosphohydrolase,
http://linkedlifedata.com/resource/pubmed/chemical/PTEN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoric Monoester Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0950-9232
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
24
|
| pubmed:volume |
17
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1557-65
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9794233-Carcinoma, Small Cell,
pubmed-meshheading:9794233-Chromosome Mapping,
pubmed-meshheading:9794233-DNA Mutational Analysis,
pubmed-meshheading:9794233-Gene Deletion,
pubmed-meshheading:9794233-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:9794233-Genes, Tumor Suppressor,
pubmed-meshheading:9794233-Humans,
pubmed-meshheading:9794233-Loss of Heterozygosity,
pubmed-meshheading:9794233-Lung Neoplasms,
pubmed-meshheading:9794233-Mutation,
pubmed-meshheading:9794233-PTEN Phosphohydrolase,
pubmed-meshheading:9794233-Phosphoric Monoester Hydrolases,
pubmed-meshheading:9794233-Point Mutation,
pubmed-meshheading:9794233-Polymorphism, Single-Stranded Conformational,
pubmed-meshheading:9794233-Protein Tyrosine Phosphatases,
pubmed-meshheading:9794233-Pseudogenes,
pubmed-meshheading:9794233-Tumor Cells, Cultured,
pubmed-meshheading:9794233-Tumor Suppressor Proteins
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Mutation analysis of the PTEN/MMAC1 gene in lung cancer.
|
| pubmed:affiliation |
Hamon Center for Therapeutic Oncology Research, University of Texas, Southwestern Medical Center, Dallas 75235, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|