|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1998-11-23
|
|
pubmed:abstractText |
Our previous studies demonstrated that matrix metalloproteinase (MMP-9) levels were significantly higher in human glioblastoma tissue samples than in low-grade brain tumors and normal brain tissue (Rao et al., Cancer Res. 53, 2208-2211, 1993). In the present study, we measured the levels of MMP-2 and MMP-9 during the growth of glial tumors in nude mice by intracerebral injection of glioblastoma cells. Using gelatin zymography, densitometry, and an enzyme-linked immunosorbent assay, we found that the enzyme activity and protein count of MMP-2 and MMP-9 were a respective 3- to 10- and 2- to 30-fold higher in tumors at day 14 and 28 than in normal tissue. Immunohistochemical staining for MMP-9 showed strong immunoreactivity in tumor cells and the staining intensity was much higher at day 28, compared to day 14. These results suggest that upregulation of MMP-9 plays a major role in the glioma tumor growth in vivo.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0006-291X
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright 1998 Academic Press.
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
20
|
|
pubmed:volume |
251
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
632-6
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:9792825-Animals,
pubmed-meshheading:9792825-Cell Division,
pubmed-meshheading:9792825-Collagenases,
pubmed-meshheading:9792825-Disease Progression,
pubmed-meshheading:9792825-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9792825-Female,
pubmed-meshheading:9792825-Gelatinases,
pubmed-meshheading:9792825-Glioblastoma,
pubmed-meshheading:9792825-Humans,
pubmed-meshheading:9792825-Immunohistochemistry,
pubmed-meshheading:9792825-Matrix Metalloproteinase 2,
pubmed-meshheading:9792825-Matrix Metalloproteinase 9,
pubmed-meshheading:9792825-Metalloendopeptidases,
pubmed-meshheading:9792825-Mice,
pubmed-meshheading:9792825-Mice, Nude,
pubmed-meshheading:9792825-Molecular Weight,
pubmed-meshheading:9792825-Time Factors,
pubmed-meshheading:9792825-Transplantation, Heterologous
|
|
pubmed:year |
1998
|
|
pubmed:articleTitle |
Elevated levels of Mr 92,000 type IV collagenase during tumor growth in vivo.
|
|
pubmed:affiliation |
Department of Neurosurgery, Department of Neuro-Oncology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas, 77030, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
