Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1998-12-17
pubmed:abstractText
Open reading frames (6116) of the budding yeast Saccharomyces cerevisiae were PCR-amplified from genomic DNA using 12,232 primers specific to the ends of the coding sequences; the success rate of amplification was 97%. PCR-products were made accessible to hybridization by being arrayed at very high density on solid support media using various robotic devices. Probes made from total RNA preparations were hybridized for the analysis of the transcriptional activity of yeast under various growth conditions and of different strains. Experimental factors that proved critical to the performance, such as different RNA isolation procedures and the assessment of hybridization results, for example, were investigated in detail. Various software tools were developed that permit convenient handling and sound analysis of the large data quantities obtained from transcriptional profiling studies. Comprehensive arrays are being distributed within the European Yeast Functional Analysis Network (EUROFAN) and beyond.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0749-503X
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1209-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9791892-Blotting, Western, pubmed-meshheading:9791892-DNA Primers, pubmed-meshheading:9791892-DNA Probes, pubmed-meshheading:9791892-Electrophoresis, Agar Gel, pubmed-meshheading:9791892-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:9791892-Enzymes, pubmed-meshheading:9791892-Gene Expression Regulation, Fungal, pubmed-meshheading:9791892-Image Processing, Computer-Assisted, pubmed-meshheading:9791892-Nucleic Acid Hybridization, pubmed-meshheading:9791892-Open Reading Frames, pubmed-meshheading:9791892-Phenol, pubmed-meshheading:9791892-Polymerase Chain Reaction, pubmed-meshheading:9791892-RNA, Fungal, pubmed-meshheading:9791892-Robotics, pubmed-meshheading:9791892-Saccharomyces cerevisiae, pubmed-meshheading:9791892-Sensitivity and Specificity, pubmed-meshheading:9791892-Transcription, Genetic
pubmed:year
1998
pubmed:articleTitle
Transcriptional profiling on all open reading frames of Saccharomyces cerevisiae.
pubmed:affiliation
Molecular-Genetic Genome Analysis Group, Deutsches Krebsforschungszentrum, Heidelberg, Germany. n.hauser@dkfz-heidelberg.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't