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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1998-11-24
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pubmed:abstractText |
The anti-common gamma chain (gammac) mAb CP.B8 is shown to inhibit interleukin 4 (IL-4)-dependent proliferation of phytohemagglutinin (PHA) activated T cells noncompetitively with respect to cytokine by blocking the IL-4-induced heterodimerization of IL-4Ralpha and gammac receptor chains. Affinities for the binding of IL-4 to Cos-7 cells transfected with huIL-4Ralpha, and to PHA blasts expressing both IL-4Ralpha and gammac, were used to estimate the affinity of the key interaction between gammac and the binary IL-4Ralpha.IL-4 complex on the cell surface. This affinity was defined in terms of the dimensionless ratio [IL-4Ralpha.IL-4.gammac]/[IL-4Ralpha.IL-4], which we designate KR. The results show that on PHA blasts this interaction is relatively weak; KR approximately 9, implying that approximately 10% of the limiting IL-4Ralpha chain remains free of gammac even at saturating concentrations of IL-4. This quantitative treatment establishes KR as a key measure of the coupling between ligand binding and receptor activation, providing a basis for functional distinctions between different receptors that are activated by ligand-induced receptor dimerization.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-1330105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-1535167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-1567539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-1948064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-2158859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-2879554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-3496417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-7484461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-7529123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-7678333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-7834741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-8013654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-8194466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-8262056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-8266078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-8395346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-8449901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-8634300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-8798580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-8887542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-8976555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-8992992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-9002949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789059-9130652
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13165-70
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9789059-Animals,
pubmed-meshheading:9789059-Antibodies, Monoclonal,
pubmed-meshheading:9789059-Cell Membrane,
pubmed-meshheading:9789059-Cells, Cultured,
pubmed-meshheading:9789059-Humans,
pubmed-meshheading:9789059-Interleukin-4,
pubmed-meshheading:9789059-Kinetics,
pubmed-meshheading:9789059-Lymphocyte Activation,
pubmed-meshheading:9789059-Mice,
pubmed-meshheading:9789059-Models, Immunological,
pubmed-meshheading:9789059-Receptor Cross-Talk,
pubmed-meshheading:9789059-Receptors, Cytokine,
pubmed-meshheading:9789059-Receptors, Interleukin-4,
pubmed-meshheading:9789059-T-Lymphocytes
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pubmed:year |
1998
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pubmed:articleTitle |
Interaction affinity between cytokine receptor components on the cell surface.
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pubmed:affiliation |
Biogen, Inc., 14 Cambridge Center, Cambridge, MA 02142, USA.
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pubmed:publicationType |
Journal Article
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