Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1998-11-24
pubmed:abstractText
Protein acetylation has been implicated in the regulation of HIV-1 gene transcription. Here, we have exploited the activities of four native histone acetyltransferase (HAT) complexes from yeast to directly test whether acetylation regulates HIV-1 transcription in vitro. HAT activities acetylating either histone H3 (SAGA, Ada, and NuA3) or H4 (NuA4) stimulate HIV-1 transcription from preassembled nucleosomal templates in an acetyl CoA-dependent manner. HIV-1 transcription from histone-free DNA is not affected by the HATs, indicating that these activities function in a chromatin-specific fashion. For Ada and NuA4, we demonstrate that acetylation of only histone proteins mediates enhanced transcription, suggesting that these complexes facilitate transcription at least in part by modifying histones. To address a potential mechanism by which HAT complexes stimulate transcription, we performed a restriction enzyme accessibility analysis. Each of the HATs increases the cutting efficiencies of restriction endonucleases targeting the HIV-1 chromatin templates in a manner not requiring transcription, suggesting that histone acetylation leads to nucleosome remodeling.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-1332230, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-1638630, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-2838379, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-2996776, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-3044607, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-3052270, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-7734837, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8168481, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8343956, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8344262, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8413201, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8458576, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8562915, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8601308, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8605881, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8684459, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8887561, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8945521, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8967953, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-8980232, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9139820, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9139821, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9149532, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9150133, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9150134, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9150135, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9150136, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9150137, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9171359, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9224714, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9267036, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9285713, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9288740, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9296499, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9311776, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9407026, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9582280, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9674426, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9697775, http://linkedlifedata.com/resource/pubmed/commentcorrection/9789016-9759497
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12924-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Purified histone acetyltransferase complexes stimulate HIV-1 transcription from preassembled nucleosomal arrays.
pubmed:affiliation
Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't