| pubmed-article:9788950 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9788950 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:9788950 | lifeskim:mentions | umls-concept:C0026018 | lld:lifeskim |
| pubmed-article:9788950 | lifeskim:mentions | umls-concept:C0016315 | lld:lifeskim |
| pubmed-article:9788950 | lifeskim:mentions | umls-concept:C1707520 | lld:lifeskim |
| pubmed-article:9788950 | lifeskim:mentions | umls-concept:C0150312 | lld:lifeskim |
| pubmed-article:9788950 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:9788950 | pubmed:dateCreated | 1998-12-4 | lld:pubmed |
| pubmed-article:9788950 | pubmed:abstractText | Fluorescence correlation microscopy (FCM), the combination of fluorescence correlation spectroscopy (FCS) and digital microscopy (Brock and Jovin, 1998. Cell. Mol. Biol. 44:847-856), has been implemented for measuring molecular diffusion and association in living cells with explicit consideration of autocorrelations arising from autofluorescence. Autofluorescence excited at 532 nm colocalizes with mitochondria, has flavin-like spectral characteristics, exhibits relaxation times characteristic for the diffusion of high-molecular-weight proteins, and depends on the incubation conditions of the cells. These time- and location-dependent properties preclude the assignment of universal background parameters. The lower limit for detection of microinjected dextran molecules labeled with the carboxymethylindocyanine dye Cy3 was a few thousand molecules per cell, and the diffusion constant of 1.7 x 10(-7) cm2/s agreed well with values measured with other methods. Based on the fluorescence signal per molecule (fpm) and the molecule number derived from autocorrelation analysis, a new method is devised to define intracellular association states. We conclude that FCM is a powerful, noninvasive method for probing molecular interactions in femtoliter volume elements within defined subcellular locations in living cells. | lld:pubmed |
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| pubmed-article:9788950 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9788950 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9788950 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9788950 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9788950 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:9788950 | pubmed:issn | 0006-3495 | lld:pubmed |
| pubmed-article:9788950 | pubmed:author | pubmed-author:JovinT MTM | lld:pubmed |
| pubmed-article:9788950 | pubmed:author | pubmed-author:BrockRR | lld:pubmed |
| pubmed-article:9788950 | pubmed:author | pubmed-author:HinkM AMA | lld:pubmed |
| pubmed-article:9788950 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9788950 | pubmed:volume | 75 | lld:pubmed |
| pubmed-article:9788950 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9788950 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9788950 | pubmed:pagination | 2547-57 | lld:pubmed |
| pubmed-article:9788950 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:9788950 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9788950 | pubmed:articleTitle | Fluorescence correlation microscopy of cells in the presence of autofluorescence. | lld:pubmed |
| pubmed-article:9788950 | pubmed:affiliation | Department of Molecular Biology, Max Planck Institute of Biophysical Chemistry, D-37077 Göttingen, Germany. | lld:pubmed |
| pubmed-article:9788950 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9788950 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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