Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1998-12-4
pubmed:abstractText
Fluorescence correlation microscopy (FCM), the combination of fluorescence correlation spectroscopy (FCS) and digital microscopy (Brock and Jovin, 1998. Cell. Mol. Biol. 44:847-856), has been implemented for measuring molecular diffusion and association in living cells with explicit consideration of autocorrelations arising from autofluorescence. Autofluorescence excited at 532 nm colocalizes with mitochondria, has flavin-like spectral characteristics, exhibits relaxation times characteristic for the diffusion of high-molecular-weight proteins, and depends on the incubation conditions of the cells. These time- and location-dependent properties preclude the assignment of universal background parameters. The lower limit for detection of microinjected dextran molecules labeled with the carboxymethylindocyanine dye Cy3 was a few thousand molecules per cell, and the diffusion constant of 1.7 x 10(-7) cm2/s agreed well with values measured with other methods. Based on the fluorescence signal per molecule (fpm) and the molecule number derived from autocorrelation analysis, a new method is devised to define intracellular association states. We conclude that FCM is a powerful, noninvasive method for probing molecular interactions in femtoliter volume elements within defined subcellular locations in living cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-1347277, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-1420920, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-15157441, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-1527376, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-17023344, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-2085652, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-220325, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-2321027, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-2340776, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-2371284, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-2423529, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-2454930, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-2458365, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-265522, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-2720061, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-2730907, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-3263271, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-3355826, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-3474634, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-3663831, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-3918045, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-438504, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-4818131, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-556653, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-6207019, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-6233965, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-6877390, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-7534502, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-7649270, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-7696520, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-8189447, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-8298032, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-8303295, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-831279, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-8337255, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-8526912, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-8537958, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-8648627, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-8673464, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-8804624, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-8910200, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-8917500, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-8917501, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-9083691, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-9276758, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-9278050, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-9288971, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-9342306, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-9362560, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-974223, http://linkedlifedata.com/resource/pubmed/commentcorrection/9788950-9764751
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2547-57
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Fluorescence correlation microscopy of cells in the presence of autofluorescence.
pubmed:affiliation
Department of Molecular Biology, Max Planck Institute of Biophysical Chemistry, D-37077 Göttingen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't