Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
44
pubmed:dateCreated
1998-12-1
pubmed:abstractText
The SCL gene, also known as tal-1, encodes a basic helix-loop-helix transcription factor that is pivotal for the normal development of all hematopoietic lineages. SCL is expressed in committed erythroid, mast, and megakaryocytic cells as well as in hematopoietic stem cells. Nothing is known about the regulation of SCL transcription in mast cells, and in other lineages GATA-1 is the only tissue-specific transcription factor recognized to regulate the SCL gene. We have therefore analyzed the molecular mechanisms underlying SCL expression in mast cells. In this paper, we demonstrate that SCL promoter 1a was regulated by GATA-1 together with Sp1 and Sp3 in a manner similar to the situation in erythroid cells. However, SCL promoter 1b was strongly active in mast cells, in marked contrast to the situation in erythroid cells. Full activity of promoter 1b was dependent on ETS and Sp1/3 motifs. Transcription factors PU.1, Elf-1, Sp1, and Sp3 were all present in mast cell extracts, bound to promoter 1b and transactivated promoter 1b reporter constructs. These data provide the first evidence that the SCL gene is a direct target for PU.1, Elf-1, and Sp3.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
273
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29032-42
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed-meshheading:9786909-Animals, pubmed-meshheading:9786909-Base Sequence, pubmed-meshheading:9786909-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:9786909-Cell Line, pubmed-meshheading:9786909-DNA Footprinting, pubmed-meshheading:9786909-DNA Primers, pubmed-meshheading:9786909-DNA-Binding Proteins, pubmed-meshheading:9786909-Gene Expression Regulation, pubmed-meshheading:9786909-Mast Cells, pubmed-meshheading:9786909-Mice, pubmed-meshheading:9786909-Mutagenesis, Site-Directed, pubmed-meshheading:9786909-Nuclear Proteins, pubmed-meshheading:9786909-Promoter Regions, Genetic, pubmed-meshheading:9786909-Proto-Oncogene Proteins, pubmed-meshheading:9786909-Trans-Activators, pubmed-meshheading:9786909-Transcription, Genetic, pubmed-meshheading:9786909-Transcription Factors
pubmed:year
1998
pubmed:articleTitle
Transcriptional regulation of the stem cell leukemia gene by PU.1 and Elf-1.
pubmed:affiliation
University of Cambridge, Department of Haematology, Medical Research Council Centre, Hills Road, Cambridge CB2 2QH, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't