Linked Life Data

9786193

Source:http://linkedlifedata.com/resource/pubmed/id/9786193

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-1-19
pubmed:databankReference
pubmed:abstractText
The type III secretion system of Salmonella pathogenicity island 2 (SPI-2) is required for systemic infection of this pathogen in mice. Cloning and sequencing of a central region of SPI-2 revealed the presence of genes encoding putative chaperones and effector proteins of the secretion system. The predicted products of the sseB, sseC and sseD genes display weak but significant similarity to amino acid sequences of EspA, EspD and EspB, which are secreted by the type III secretion system encoded by the locus of enterocyte effacement of enteropathogenic Escherichia coli. The transcriptional activity of an sseA::luc fusion gene was shown to be dependent on ssrA, which is required for the expression of genes encoding components of the secretion system apparatus. Strains carrying nonpolar mutations in sseA, sseB or sseC were severely attenuated in virulence, strains carrying mutations in sseF or sseG were weakly attenuated, and a strain with a mutation in sseE had no detectable virulence defect. These phenotypes were reflected in the ability of mutant strains to grow within a variety of macrophage cell types: strains carrying mutations in sseA, sseB or sseC failed to accumulate, whereas the growth rates of strains carrying mutations in sseE, sseF or sseG were only modestly reduced. These data suggest that, in vivo, one of the functions of the SPI-2 secretion system is to enable intracellular bacterial proliferation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
163-74
pubmed:dateRevised
2009-9-1
pubmed:meshHeading
pubmed-meshheading:9786193-Acetyltransferases, pubmed-meshheading:9786193-Amino Acid Sequence, pubmed-meshheading:9786193-Animals, pubmed-meshheading:9786193-Artificial Gene Fusion, pubmed-meshheading:9786193-Bacterial Proteins, pubmed-meshheading:9786193-Bacteriophages, pubmed-meshheading:9786193-Cloning, Molecular, pubmed-meshheading:9786193-DNA, Bacterial, pubmed-meshheading:9786193-Female, pubmed-meshheading:9786193-Genes, Bacterial, pubmed-meshheading:9786193-Genes, Reporter, pubmed-meshheading:9786193-Macrophages, Peritoneal, pubmed-meshheading:9786193-Mice, pubmed-meshheading:9786193-Mice, Inbred BALB C, pubmed-meshheading:9786193-Molecular Chaperones, pubmed-meshheading:9786193-Molecular Sequence Data, pubmed-meshheading:9786193-Salmonella Infections, Animal, pubmed-meshheading:9786193-Salmonella typhimurium, pubmed-meshheading:9786193-Sequence Analysis, DNA, pubmed-meshheading:9786193-Transcription, Genetic, pubmed-meshheading:9786193-Virulence
pubmed:year
1998
pubmed:articleTitle
Genes encoding putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2 are required for bacterial virulence and proliferation in macrophages.
pubmed:affiliation
Lehrstuhl für Bakteriologie, Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Munich, Germany.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't