Linked Life Data

9786177

Source:http://linkedlifedata.com/resource/pubmed/id/9786177

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1998-12-17
pubmed:abstractText
Pituitary adenylate cyclase activating polypeptide (PACAP), a member of the secretin/glucagon/vasoactive intestinal polypeptide family of peptides, exists as 38-residue (PACAP 38) and truncated 27-residue (PACAP 27) forms, which play various roles in mammals. Recently, we isolated and characterized PACAPs with sequences very similar to that of tetrapod PACAP from the brains of two teleosts, the blue-spotted stargazer Gnathagnus elongatus and the stargazer Uranoscopus japonicus, and located PACAP-like immunoreactivities in the preoptic area, neurohypophysis and medulla oblongata of both species. In this study, PACAP-like immunoreactivity in the brain of an elasmobranch, the stingray Dasyatis akajei, was examined by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and Western blotting analysis and its distribution in the hypothalamo-pituitary region was studied immunohistochemically using the peroxidase-antiperoxidase method. The anti-PACAP 27 serum reacted with a single band of whole brain extract with a molecular weight of ca 5000. PACAP-like immunoreactive (LI) neuronal cell bodies were found in the nucleus medius hypothalami and PACAP-LI nerve fibers and terminals were seen from the nucleus to the caudal floor of the infundibular region. These results suggest that PACAP-like peptide may be present and function as a regulatory factor in the hypothalamo-pituitary region of the elasmobranch brain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0196-9781
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1263-7
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
The localization of pituitary adenylate cyclase-activating polypeptide (PACAP)-like immunoreactivity in the hypothalamo-pituitary region of an elasmobranch, stingray, Dasyatis akajei.
pubmed:affiliation
Department of Biology, Faculty of Science, Toyama University, Gofuku, Japan. kmatsuda@sci.toyama-u.ac.jp
pubmed:publicationType
Journal Article