Linked Life Data

9782512

Source:http://linkedlifedata.com/resource/pubmed/id/9782512

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1999-1-4
pubmed:abstractText
Sinorhizobium meliloti 1021 and Rhizobium etli CE3 turn over nitrogen and carbon from glutamine to ammonium and CO2, respectively. Some of the ammonium released is assimilated back into glutamine, indicating that a glutamine cycle similar to that in Neurospora operates in Rhizobium. In addition, a previously unrecognized metabolic pathway in Rhizobium was discovered--namely, conversion of glutamine-carbon to gamma-hydroxybutyric acid and beta-hydroxybutyric acid. Additionally, some of the 2-oxoglutarate derived from glutamine catabolism in Rhizobium is converted to succinate in glutamine-containing medium. Both S. meliloti 1021 and R. etli CE3 oxidize succinate preferentially over glutamine when provided with both carbon sources. In contrast to Sinorhizobium meliloti 1021 and Rhizobium etli CE3, an S. meliloti double mutant that lacks both glutamine synthetase (GS) I and II preferentially oxidizes glutamine over succinate when supplied with both substrates. GSII activity is induced in wild-type S. meliloti 1021 and R. etli CE3 grown in succinate-glutamine medium, and this enzyme participates in the cycling of glutamine-carbon and -nitrogen. On the other hand, GSII activity is repressed in both micro-organisms when glutamine is the only carbon source. These findings show that, in medium containing both glutamine and succinate, glutamine synthesis helps drive the utilization of succinate. When glutamine is in excess as an energy-providing substrate its synthesis is restricted, allowing for more effective utilization of glutamine as an energy source.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
144 ( Pt 9)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2629-38
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Glutamine biosynthesis and the utilization of succinate and glutamine by Rhizobium etli and Sinorhizobium meliloti.
pubmed:affiliation
Departamento de Ecología Molecular, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't