Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1998-12-22
|
| pubmed:databankReference | |
| pubmed:abstractText |
Here we report the isolation and characterization of mouse testicular cDNAs encoding the mammalian homologue of the Xenopus germ cell-specific nucleic acid-binding protein FRGY2 (mRNP3+4), hereafter designated MSY2. MSY2 is a member of the Y box multigene family of proteins; it contains the cold shock domain that is highly conserved among all Y box proteins and four basic/aromatic islands that are closely related to the other known germline Y box proteins from Xenopus, FRGY2, and goldfish, GFYP2. Msy2 undergoes alternative splicing to yield alternate N-terminal regions upstream of the cold shock domain. Although MSY2 is a member of a large family of nucleic acid-binding proteins, Southern blotting detects only a limited number of genomic DNA fragments, suggesting that Msy2 is a single copy gene. By Northern blotting and immunoblotting, MSY2 appears to be a germ cell-specific protein in the testis. Analysis of Msy2 mRNA expression in prepubertal and adult mouse testes, and in isolated populations of germ cells, reveals maximal expression in postmeiotic round spermatids, a cell type with abundant amounts of stored messenger ribonucleoproteins. In the ovary, MSY2 is present exclusively in diplotene-stage and mature oocytes. MSY2 is maternally inherited in the one-cell-stage embryo but is not detected in the late two-cell-stage embryo. This loss of MSY2 is coincident with the bulk degradation of maternal mRNAs in the two-cell embryo.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0006-3363
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
59
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1266-74
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9780336-Alternative Splicing,
pubmed-meshheading:9780336-Amino Acid Sequence,
pubmed-meshheading:9780336-Animals,
pubmed-meshheading:9780336-Base Sequence,
pubmed-meshheading:9780336-Blotting, Southern,
pubmed-meshheading:9780336-Female,
pubmed-meshheading:9780336-Gene Expression Regulation, Developmental,
pubmed-meshheading:9780336-Germ Cells,
pubmed-meshheading:9780336-Goldfish,
pubmed-meshheading:9780336-Male,
pubmed-meshheading:9780336-Mice,
pubmed-meshheading:9780336-Molecular Sequence Data,
pubmed-meshheading:9780336-Oocytes,
pubmed-meshheading:9780336-RNA-Binding Proteins,
pubmed-meshheading:9780336-Restriction Mapping,
pubmed-meshheading:9780336-Sequence Analysis,
pubmed-meshheading:9780336-Spermatogenesis,
pubmed-meshheading:9780336-Spermatozoa,
pubmed-meshheading:9780336-Testis,
pubmed-meshheading:9780336-Xenopus
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Mammalian male and female germ cells express a germ cell-specific Y-Box protein, MSY2.
|
| pubmed:affiliation |
Department of Biology, Tufts University, Medford, Massachusetts 02155, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|