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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1998-12-30
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pubmed:abstractText |
Most immunoelectron microscopy techniques used for ultrastructural analyses of virus-infected plant tissues significantly compromise cellular membranous structures as well as overall contrast and resolution of the image. Here, we describe a protocol which avoids these flaws but retains full antigenicity of the sample. A direct comparison of the conventional and the improved electron immunostaining procedures is presented using tobacco and Arabidopsis thaliana plants infected with turnip vein clearing virus.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0166-0934
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
223-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9779623-Arabidopsis,
pubmed-meshheading:9779623-Brassica,
pubmed-meshheading:9779623-Microscopy, Immunoelectron,
pubmed-meshheading:9779623-Plants,
pubmed-meshheading:9779623-Plants, Toxic,
pubmed-meshheading:9779623-Tissue Embedding,
pubmed-meshheading:9779623-Tissue Fixation,
pubmed-meshheading:9779623-Tobacco,
pubmed-meshheading:9779623-Tobamovirus
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pubmed:year |
1998
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pubmed:articleTitle |
Preservation of plant cell ultrastructure during immunolocalization of virus particles.
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pubmed:affiliation |
Department of Plant Sciences, University of Arizona, Tucson 85721, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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