Linked Life Data

9777353

Source:http://linkedlifedata.com/resource/pubmed/id/9777353

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1998-12-10
pubmed:abstractText
The nucleotide sequence of a 1000-bp fragment of the Escherichia coli chromosome located between genes metE and udp and including the promoter region of the udp gene was determined. Multiple binding sites for the CytR and CRP proteins were identified outside the canonical udp gene promoter. A set of deletion variants with the truncated regulatory region of the udp gene was isolated based on plasmids pSKII and pJEL250. The level of CytR regulation of the udp gene was shown to depend on the size of the regulatory region relative to the transcription initiation site. On the basis of these data, it is concluded that additional binding sites for the CytR protein located in the regulatory region are functionally active in the regulation of udp gene expression. This conclusion has been confirmed by properties of the udp264 promoter mutant, which contains a deletion covering the main CytR binding site within the canonical promoter. Irrespective of the deletion, the expression of the udp gene in mutant udpP264 retains the dependence on the allelic state of the cytR gene. The CytR protein was shown to act as a transcription repressor or activator, depending on the configuration of the promoter and on the relative location and quantity of binding sites for CytR and CRP proteins.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0016-6758
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1063-72
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
[Role of additional binding sites for the CytR protein in the regulation of Escherichia coli udp gene expression].
pubmed:affiliation
State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia. lab25@vnigen.msk.su
pubmed:publicationType
Journal Article, English Abstract