Linked Life Data

9776750

Source:http://linkedlifedata.com/resource/pubmed/id/9776750

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1998-12-23
pubmed:abstractText
In this paper, we present evidence that an efficient Rho-dependent terminator can be created by introducing a simple (AG/TC) n DNA repeat into a transcription unit. The Rho termination activity in vivo and in vitro is dependent on the length and the orientation of the insert. The transcription of at least 30 bp of the (AG/TC) n repeat in the orientation encoding the (rUrC) n sequence on the transcript leads to Rho-dependent termination at a downstream non-terminator site. Our results indicate that the high efficiency of this artificial Rho-dependent terminator is due to optimal interactions between the (rUrC) n RNA sequence and Rho protein. Thus, our findings strongly suggest that an adequate loading site is the primary determinant for Rho termination activity and provide a more defined system for future investigations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4895-900
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
A simple polypyrimidine repeat acts as an artificial Rho-dependent terminator in vivo and in vitro.
pubmed:affiliation
Centre de Biophysique Moléculaire, CNRS, rue Charles Sadron, 45071 Orléans cédex 2, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't