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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1998-10-27
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pubmed:abstractText |
We genetically engineered human myelomonocytic KG-I cells by introducing cDNA of murine interleukin-18 receptor (MuIL-18R) and established human cells which were capable of responding to MuIL-18. These cells expressed larger number of MuIL-18R (> 13,000 sites/cell) than intrinsic human IL-18 receptor (HuIL-18R) (< 2,500 sites/cell). And the cells responded to MuIL-18 as well as to HuIL-18 in a dose-dependent manner, and produced large amounts of interferon-gamma (IFN-gamma). We could estimate the amount of murine IL-18 based on the amounts of IFN-gamma produced by these cells. The stoichiometry was observed up to 150 ng/ml of MuIL-18. By using these cells, a large amount of MuIL-18 (448 +/- 89.2 ng/ml) was detected in sera of Propionibacterium acnes (P. acnes)/lipopolysaccharide (LPS)-treated endotoxic mice (the same conditions in which IL-18 was first identified). These cells provide us with a useful tool for determining the bioactivity of MuIL-18.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/IL18R1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Il18r1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-18,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-18 Receptor alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-18,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1759
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
217
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
97-102
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9776579-Animals,
pubmed-meshheading:9776579-Biological Assay,
pubmed-meshheading:9776579-DNA, Complementary,
pubmed-meshheading:9776579-Endotoxemia,
pubmed-meshheading:9776579-Genetic Vectors,
pubmed-meshheading:9776579-Humans,
pubmed-meshheading:9776579-Interferon-gamma,
pubmed-meshheading:9776579-Interleukin-18,
pubmed-meshheading:9776579-Interleukin-18 Receptor alpha Subunit,
pubmed-meshheading:9776579-Lipopolysaccharides,
pubmed-meshheading:9776579-Mice,
pubmed-meshheading:9776579-Mice, Inbred C57BL,
pubmed-meshheading:9776579-Monocytes,
pubmed-meshheading:9776579-Propionibacterium acnes,
pubmed-meshheading:9776579-Protein Binding,
pubmed-meshheading:9776579-Receptors, Interleukin,
pubmed-meshheading:9776579-Receptors, Interleukin-18,
pubmed-meshheading:9776579-Recombinant Fusion Proteins,
pubmed-meshheading:9776579-Signal Transduction,
pubmed-meshheading:9776579-Tumor Cells, Cultured
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pubmed:year |
1998
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pubmed:articleTitle |
Establishment of the cells useful for murine interleukin-18 bioassay by introducing murine interleukin-18 receptor cDNA into human myelomonocytic KG-1 cells.
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pubmed:affiliation |
Fujisaki Institute, Hayashibara Biochemical Laboratories Inc., Okayama, Japan.
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pubmed:publicationType |
Journal Article
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