Linked Life Data

9776234

Source:http://linkedlifedata.com/resource/pubmed/id/9776234

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1998-12-22
pubmed:abstractText
Polyomaviruses induce tumours of different histological types when inoculated into experimental animals, but their aetiological role in the development of malignant tumours in humans remains questionable. We developed a degenerate PCR assay in an attempt to identify additional, presently unknown human polyomavirus types which may be involved in the malignant transformation of human tissues. Degenerate oligonucleotide primers were deduced from four different conserved amino acid motifs in the highly conserved viral capsid protein, VP1. Three different sets of primers were included for the each test. Bladder carcinomas, Hodgkin's lymphomas, meningiomas, Kaposi-tumours and -cell lines were analysed. No polyomavirus DNA sequences could be detected. A comparative analysis led to the recognition of the presence of SV40 DNA sequences in more than 200 vectors available in the EMBL and Genbank Databanks and commonly used in laboratories worldwide. The majority of primers used to detect polyomavirus sequences in human tumours are distributed throughout these regions also present in the vectors. Only a small stretch of 286bp in the overlapping region of the VP1, VP2 and VP3 genes is not present in the vector sequences. We propose to use this region for the design of additional non-contamination primers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-5149
pubmed:author
pubmed:issnType
Print
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
A broad spectrum PCR method for the detection of polyomaviruses and avoidance of contamination by cloning vectors.
pubmed:affiliation
Deutsches Krebsforschungszentrum, Abteilung Tumorvirus-Charakterisierung, Heidelberg, Germany.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't