Linked Life Data

977594

Source:http://linkedlifedata.com/resource/pubmed/id/977594

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1977-1-3
pubmed:abstractText
A naturally occurring fluorescent compound, beta-parinaric acid, was employed as a probe to measure the effects of temperature changes on plasma membrenes, microsomes, and mitochondria and on their respective lipids after isolation form LM cells grown in suspension culture. A computer-centered spectrofluorimenter simultaneously measured the absorbance, absorbance-corrected fluorescence, and relative fluorescence efficiency of beta-parinaric acid incorporated into the membranes or isolated membrane lipids. These parameters were measured as a function of temperature. The probe revealed five characteristic breaks or changes in slope with both the plasma membranes as well as their extracted lipids. These discontinuities occurred at approximately 18, 23, 31, 38, and 43 degrees. The other isolated subcellular organelles, microsomes, and mitochondria, as well as their respective isolated lipids, exhibited approximately the same characteristic temperatures (+/- 1 degree) as plasma membranes. Thus, these data negate one criterion of the theory that an asymmetric distribution of characteristic temperatures exist across the membranes of LM cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
251
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6739-46
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Use of beta-parinaric acid, a novel fouorimetric probe, to determine characteristic temperatures of membranes and membrane lipids from cultured animal cells.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.