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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1998-11-5
|
| pubmed:abstractText |
The immunogenicity and protective efficacy of DNA-based vaccination with plasmids encoding the membrane proteins prM and E of the flavivirus Murray Valley encephalitis virus (MVE) were investigated. Gene gun-mediated intradermal delivery of DNA encoding the prM and E proteins elicited long-lived, virus-neutralising antibody responses in three inbred strains of mice and provided protection from challenge with a high titer inoculum of MVE. Intramuscular DNA vaccination by needle injection also induced MVE-specific antibodies that conferred resistance to challenge with live virus but failed to reduce virus infectivity in vitro. The two routes of DNA-based vaccination with prM and E encoding plasmids resulted in humoral immunty with distinct IgG subtypes. MVE-specific IgG1 antibodies were always prevalent after intradermal DNA vaccination via a gene gun but not detected when mice were immunised with DNA by the intramuscular route or infected with live virus. We also tested a Semliki Forest virus replicon as vector for a flavivirus prM and E protein-based subunit vaccine. Single-cycle infections in mice vaccinated with packaged recombinant replicon particles elicited durable, MVE-specific, and virus-neutralising antibody responses.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/glycoprotein E, Flavivirus,
http://linkedlifedata.com/resource/pubmed/chemical/prM protein, Flavivirus
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0042-6822
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1998 Academic Press.
|
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
250
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
151-63
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9770429-Animals,
pubmed-meshheading:9770429-Antibodies, Viral,
pubmed-meshheading:9770429-Biolistics,
pubmed-meshheading:9770429-COS Cells,
pubmed-meshheading:9770429-Encephalitis, Arbovirus,
pubmed-meshheading:9770429-Encephalitis Virus, Murray Valley,
pubmed-meshheading:9770429-Genetic Vectors,
pubmed-meshheading:9770429-Immunoglobulin G,
pubmed-meshheading:9770429-Mice,
pubmed-meshheading:9770429-Mice, Inbred Strains,
pubmed-meshheading:9770429-Neutralization Tests,
pubmed-meshheading:9770429-Plasmids,
pubmed-meshheading:9770429-Recombinant Fusion Proteins,
pubmed-meshheading:9770429-Semliki forest virus,
pubmed-meshheading:9770429-Vaccination,
pubmed-meshheading:9770429-Vaccines, DNA,
pubmed-meshheading:9770429-Viral Envelope Proteins,
pubmed-meshheading:9770429-Viral Vaccines
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
DNA-based and alphavirus-vectored immunisation with prM and E proteins elicits long-lived and protective immunity against the flavivirus, Murray Valley encephalitis virus.
|
| pubmed:affiliation |
John Curtin School of Medical Research, The Australian National University, Canberra, ACT, 2601, Australia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|