Linked Life Data

9769172

Source:http://linkedlifedata.com/resource/pubmed/id/9769172

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-11-16
pubmed:abstractText
Interaction between germ cells and the supporting somatic cells guides many of the differentiative processes of gametogenesis. The expression pattern of the Pem homeobox gene suggests that it may mediate specific inductive events in murine reproductive tissues. During gestation, Pem is expressed in migrating and early postmigratory primordial germ cells, as well as in all embryo-derived extraembryonic membranes. Pem expression ceases in the germline after Embryonic Day 14 in both sexes and then reappears postnatally in the supporting cells of the gonad. In mature mice, Pem is produced by testicular Sertoli cells during stages VI-VIII of spermatogenesis and transiently by ovarian granulosa cells lining periovulatory follicles. Despite this tightly regulated reproductive expression pattern, mice with a targeted mutation in Pem have normal fecundity, with no detectable alteration in extraembryonic testicular or ovarian development or function. We also show that Pem is expressed throughout embryonic and adult development in a subset of a tissue-specific class of macrophages, Kupffer cells, as well as in a localized fraction of cells in macrophage cell lines. Although the number of Pem-positive Kupffer cells increases in mice treated with lipopolysaccharide, loss of Pem does not detectably interfere with the cells' ability to induce iNOS expression, demonstrating this Kupffer cell function does not require Pem. No differences were observed between Pem-knockout mice in 129, C57BL6/J, or mixed genetic backgrounds. Together, these data show that Pem is dispensable for embryonic and postnatal development, gonadal function, and Kupffer cell activation, perhaps due to compensatory expression of a similar homeobox gene.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0012-1606
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Academic Press.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
202
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
196-214
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9769172-Animals, pubmed-meshheading:9769172-Female, pubmed-meshheading:9769172-Fertility, pubmed-meshheading:9769172-Gametogenesis, pubmed-meshheading:9769172-Gene Expression Regulation, Developmental, pubmed-meshheading:9769172-Gene Targeting, pubmed-meshheading:9769172-Genes, Homeobox, pubmed-meshheading:9769172-Homeodomain Proteins, pubmed-meshheading:9769172-Kupffer Cells, pubmed-meshheading:9769172-Macrophages, pubmed-meshheading:9769172-Male, pubmed-meshheading:9769172-Mice, pubmed-meshheading:9769172-Mice, Inbred C57BL, pubmed-meshheading:9769172-Mice, Knockout, pubmed-meshheading:9769172-Mice, Transgenic, pubmed-meshheading:9769172-Nitric Oxide Synthase, pubmed-meshheading:9769172-Nitric Oxide Synthase Type II, pubmed-meshheading:9769172-Ovary, pubmed-meshheading:9769172-RNA, Messenger, pubmed-meshheading:9769172-Reproduction, pubmed-meshheading:9769172-Testis, pubmed-meshheading:9769172-Transcription Factors
pubmed:year
1998
pubmed:articleTitle
Normal reproductive and macrophage function in Pem homeobox gene-deficient mice.
pubmed:affiliation
Cancer Center, Graduate Program in Biomedical Sciences, Department of Reproductive Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093-0684, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't