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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-10-28
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pubmed:abstractText |
The DNA-dependent protein kinase is a mammalian protein complex composed of Ku70, Ku80, and DNA-PKcs subunits that has been implicated in DNA double-strand break repair and V(D)J recombination. Here, by gene targeting, we have constructed a mouse with a disruption in the kinase domain of DNA-PKcs, generating an animal model completely devoid of DNA-PK activity. Our results demonstrate that DNA-PK activity is required for coding but not for signal join formation in mice. Although our DNA-PKcs defective mice closely resemble Scid mice, they differ by having elevated numbers of CD4+CD8+ thymocytes. This suggests that the Scid mice may not represent a null phenotype and may retain some residual DNA-PKcs function.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1074-7613
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pubmed:author |
pubmed-author:AmatucciA GAG,
pubmed-author:BeamishH JHJ,
pubmed-author:GelbNN,
pubmed-author:HerreraV LVL,
pubmed-author:JacksonS PSP,
pubmed-author:JeggoP APA,
pubmed-author:Marshak RothsteinAA,
pubmed-author:PriestleyAA,
pubmed-author:TaccioliG EGE,
pubmed-author:Torres ArzayusM IMI,
pubmed-author:XiangX HXH
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
355-66
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9768755-Animals,
pubmed-meshheading:9768755-B-Lymphocytes,
pubmed-meshheading:9768755-Catalysis,
pubmed-meshheading:9768755-Cell Differentiation,
pubmed-meshheading:9768755-Cells, Cultured,
pubmed-meshheading:9768755-DNA-Activated Protein Kinase,
pubmed-meshheading:9768755-DNA-Binding Proteins,
pubmed-meshheading:9768755-Embryo, Mammalian,
pubmed-meshheading:9768755-Fibroblasts,
pubmed-meshheading:9768755-Gene Targeting,
pubmed-meshheading:9768755-Genes, T-Cell Receptor,
pubmed-meshheading:9768755-Immunoglobulins,
pubmed-meshheading:9768755-Mice,
pubmed-meshheading:9768755-Mice, Inbred C57BL,
pubmed-meshheading:9768755-Mice, Transgenic,
pubmed-meshheading:9768755-Protein Structure, Tertiary,
pubmed-meshheading:9768755-Protein-Serine-Threonine Kinases,
pubmed-meshheading:9768755-Radiation Tolerance,
pubmed-meshheading:9768755-Recombination, Genetic,
pubmed-meshheading:9768755-Severe Combined Immunodeficiency,
pubmed-meshheading:9768755-T-Lymphocytes
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pubmed:year |
1998
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pubmed:articleTitle |
Targeted disruption of the catalytic subunit of the DNA-PK gene in mice confers severe combined immunodeficiency and radiosensitivity.
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pubmed:affiliation |
Department of Microbiology, Boston University School of Medicine, Massachusetts 02118, USA. taccioli@bu.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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