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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1998-11-16
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pubmed:abstractText |
Reaction centers from the photosynthetic bacterium Rhodobacter sphaeroides were oriented in phospholipid interfacial layers adsorbed to a Teflon film separating two electrolyte-filled compartments of a Teflon cell. Light-induced voltage changes were measured as a function of time across electrodes immersed in the cell compartments. The experimental system is characterized both experimentally and theoretically to relate the measured signals to the light-induced displacement currents in the reaction centers. Mathematical relations between the measured signals and the distances and geometries of the charge-transfer reactions are derived. At pH 8.0 the reaction centers were found to be oriented with approximately 60% of the population oriented with the donor facing the aqueous phase. The density of the reaction centers in the layer was approximately 10(11) cm-2, which is close to that found in the native system. Reconstitution of the secondary quinone, QB, in 90% of the RCs was achieved with an approximately 100-fold excess of ubiquinone in the vesicle preparation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-2631
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
213-25
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9767675-Electrochemistry,
pubmed-meshheading:9767675-Electron Transport,
pubmed-meshheading:9767675-Indicators and Reagents,
pubmed-meshheading:9767675-Light,
pubmed-meshheading:9767675-Lipid Bilayers,
pubmed-meshheading:9767675-Models, Theoretical,
pubmed-meshheading:9767675-Photosynthetic Reaction Center Complex Proteins,
pubmed-meshheading:9767675-Rhodobacter sphaeroides
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pubmed:year |
1998
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pubmed:articleTitle |
Charge displacements in interfacial layers containing reaction centers.
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pubmed:affiliation |
Department of Biochemistry and Biophysics, Göteborg University and Chalmers University of Technology (Medicinaregatan 9C), P.O. Box 462, SE-405 30 Göteborg, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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