Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1998-11-30
|
| pubmed:databankReference | |
| pubmed:abstractText |
Two alternatively spliced transcripts, psiHLAO1 and psiHLAO2, of a copper-containing monoamine oxidase pseudogene have been isolated from a human-liver cDNA library. The larger psiHLAO1 cDNA (2073bp) contains a 5'-flanking segment of 134bp, followed by an apparent open reading frame (ORF) of 1725bp. The deduced amino acid sequence of this ORF (574 residues) shares 81.0% similarity with the 763-residue monoamine oxidase from human placenta (HPAO) (the N-terminal 533 residues of psiHLAO1 share 86.7% similarity with HPAO). The psiHLAO1 ORF is interrupted by an in-frame stop codon corresponding to amino acid 225 and terminates within a type S(a) dimeric Alu repeat sequence. psiHLAO2 appears to be an alternatively spliced variant of psiHLAO1 that has 413 bases of psiHLAO1 excised according to the 'GT-AG' rule. The slightly longer 3' end of the psiHLAO2 transcript shows that the Alu repeat is followed by an 11-bp poly(A) tract that, in turn, is followed by an AT-rich (81%) sequence of 105bp. A reverse transcriptase-polymerase chain reaction (RT-PCR) protocol was used to confirm that both psiHLAO1 and psiHLAO2 are transcribed in human liver and placenta. A search of the expressed sequence tag (EST) database indicates that, like HPAO, psiHLAO derives also from the region 17q21 of the human genome.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0378-1119
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
220
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
71-6
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9767118-Alternative Splicing,
pubmed-meshheading:9767118-Alu Elements,
pubmed-meshheading:9767118-Amine Oxidase (Copper-Containing),
pubmed-meshheading:9767118-Amino Acid Sequence,
pubmed-meshheading:9767118-Base Sequence,
pubmed-meshheading:9767118-Blotting, Northern,
pubmed-meshheading:9767118-Chromosome Mapping,
pubmed-meshheading:9767118-Chromosomes, Human, Pair 17,
pubmed-meshheading:9767118-DNA, Complementary,
pubmed-meshheading:9767118-Dimerization,
pubmed-meshheading:9767118-Humans,
pubmed-meshheading:9767118-Liver,
pubmed-meshheading:9767118-Molecular Sequence Data,
pubmed-meshheading:9767118-Monoamine Oxidase,
pubmed-meshheading:9767118-Pseudogenes,
pubmed-meshheading:9767118-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:9767118-Transcription, Genetic
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
cDNA cloning of two splice variants of a human copper-containing monoamine oxidase pseudogene containing a dimeric Alu repeat sequence.
|
| pubmed:affiliation |
Molecular Biology Division, Department of Veterans Affairs Medical Center, 4150 Clement St., San Francisco, CA 94121, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|