| pubmed-article:9766898 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9766898 | lifeskim:mentions | umls-concept:C0017535 | lld:lifeskim |
| pubmed-article:9766898 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:9766898 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:9766898 | pubmed:dateCreated | 1998-12-14 | lld:pubmed |
| pubmed-article:9766898 | pubmed:abstractText | The development of a polymerase chain reaction (PCR) based fingerprinting method for the characterization of Giardia duodenalis isolates is described. The method uses three different PCRs; one is specific for the A ("Polish") major group of G. duodenalis isolates, another is specific for the B ("Belgian") group of isolates; and one amplifies a fragment of the glutamate dehydrogenase gene present in all G. duodenalis isolates. The PCRs perform highly sensitively on DNA from cultured trophozoites. Isolates were further characterized by restriction-fragment-length polymorphism (RFLP) analysis of the PCR products. In this way, representative isolates from the A and B groups could be grouped together into a number of subgroups. The stability of the genotypes with time and the reproducibility of the two methods were tested on cloned and subcloned lines from a number of isolates and proved to be highly satisfactory. The PCR/RFLP method was evaluated on cysts derived from a number of human patients. It is concluded that the PCR fingerprinting method described in this paper provides a reliable characterization method for Giardia isolates and has the potential to be used as a direct method of typing G. duodenalis cysts from feces. | lld:pubmed |
| pubmed-article:9766898 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9766898 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9766898 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9766898 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9766898 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:9766898 | pubmed:issn | 0932-0113 | lld:pubmed |
| pubmed-article:9766898 | pubmed:author | pubmed-author:van KnapenFF | lld:pubmed |
| pubmed-article:9766898 | pubmed:author | pubmed-author:HomanW LWL | lld:pubmed |
| pubmed-article:9766898 | pubmed:author | pubmed-author:LimperLL | lld:pubmed |
| pubmed-article:9766898 | pubmed:author | pubmed-author:GilsingMM | lld:pubmed |
| pubmed-article:9766898 | pubmed:author | pubmed-author:BentalaHH | lld:pubmed |
| pubmed-article:9766898 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9766898 | pubmed:volume | 84 | lld:pubmed |
| pubmed-article:9766898 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9766898 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9766898 | pubmed:pagination | 707-14 | lld:pubmed |
| pubmed-article:9766898 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
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| pubmed-article:9766898 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9766898 | pubmed:articleTitle | Characterization of Giardia duodenalis by polymerase-chain-reaction fingerprinting. | lld:pubmed |
| pubmed-article:9766898 | pubmed:affiliation | Microbiological Laboratory of Health Protection, National Institute of Public Health and the Environment, Bilthoven, The Netherlands. wieger.homan@rivm.nl | lld:pubmed |
| pubmed-article:9766898 | pubmed:publicationType | Journal Article | lld:pubmed |
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