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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1998-12-14
|
| pubmed:abstractText |
The development of a polymerase chain reaction (PCR) based fingerprinting method for the characterization of Giardia duodenalis isolates is described. The method uses three different PCRs; one is specific for the A ("Polish") major group of G. duodenalis isolates, another is specific for the B ("Belgian") group of isolates; and one amplifies a fragment of the glutamate dehydrogenase gene present in all G. duodenalis isolates. The PCRs perform highly sensitively on DNA from cultured trophozoites. Isolates were further characterized by restriction-fragment-length polymorphism (RFLP) analysis of the PCR products. In this way, representative isolates from the A and B groups could be grouped together into a number of subgroups. The stability of the genotypes with time and the reproducibility of the two methods were tested on cloned and subcloned lines from a number of isolates and proved to be highly satisfactory. The PCR/RFLP method was evaluated on cysts derived from a number of human patients. It is concluded that the PCR fingerprinting method described in this paper provides a reliable characterization method for Giardia isolates and has the potential to be used as a direct method of typing G. duodenalis cysts from feces.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0932-0113
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
84
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
707-14
|
| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:9766898-Adolescent,
pubmed-meshheading:9766898-Adult,
pubmed-meshheading:9766898-Aged,
pubmed-meshheading:9766898-Animals,
pubmed-meshheading:9766898-Child,
pubmed-meshheading:9766898-Child, Preschool,
pubmed-meshheading:9766898-Cloning, Molecular,
pubmed-meshheading:9766898-DNA, Protozoan,
pubmed-meshheading:9766898-DNA Fingerprinting,
pubmed-meshheading:9766898-DNA Primers,
pubmed-meshheading:9766898-Feces,
pubmed-meshheading:9766898-Giardia,
pubmed-meshheading:9766898-Giardiasis,
pubmed-meshheading:9766898-Humans,
pubmed-meshheading:9766898-Infant,
pubmed-meshheading:9766898-Middle Aged,
pubmed-meshheading:9766898-Polymerase Chain Reaction,
pubmed-meshheading:9766898-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:9766898-Restriction Mapping
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Characterization of Giardia duodenalis by polymerase-chain-reaction fingerprinting.
|
| pubmed:affiliation |
Microbiological Laboratory of Health Protection, National Institute of Public Health and the Environment, Bilthoven, The Netherlands. wieger.homan@rivm.nl
|
| pubmed:publicationType |
Journal Article
|