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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
1998-12-16
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pubmed:abstractText |
A microbiological survey of 50 retail juices was conducted in the fall of 1996. These juices were analyzed for Listeria monocytogenes, Escherichia coli O157:H7, Salmonella, coliforms, fecal coliforms, and pH. Two unpasteurized juices were positive for L. monocytogenes: an apple juice and an apple raspberry blend with a pH of 3.78 and 3.75, respectively. Three L. monocytogenes isolates were characterized. The colonies were typical for Listeria sp. on Oxford and lithium chloride-phenylethanol-moxalactam agars and were beta-hemolytic on sheep blood agar. The isolates required 5 days of incubation at 35 degrees C to produce a positive rhamnose reaction in a phenol red carbohydrate broth. This slow rhamnose utilization resulted in these isolates not being identified using the Micro-ID test strip (Organon Technika). However, the isolates were positive for L. monocytogenes using the API Listeria strip (BioMerieux) and a multiplex polymerase chain reaction for detection of the hemolysis (hyla) and invasion-associated protein (iap) genes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0362-028X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1199-202
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pubmed:dateRevised |
2000-12-18
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pubmed:meshHeading |
pubmed-meshheading:9766077-Bacteriological Techniques,
pubmed-meshheading:9766077-Beverages,
pubmed-meshheading:9766077-Culture Media,
pubmed-meshheading:9766077-DNA, Bacterial,
pubmed-meshheading:9766077-Food Contamination,
pubmed-meshheading:9766077-Fruit,
pubmed-meshheading:9766077-Hemolysis,
pubmed-meshheading:9766077-Hydrogen-Ion Concentration,
pubmed-meshheading:9766077-Listeria monocytogenes,
pubmed-meshheading:9766077-Polymerase Chain Reaction,
pubmed-meshheading:9766077-Reagent Kits, Diagnostic,
pubmed-meshheading:9766077-Sterilization
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pubmed:year |
1998
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pubmed:articleTitle |
Identification of Listeria monocytogenes from unpasteurized apple juice using rapid test kits.
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pubmed:affiliation |
U. S. Food and Drug Administration, Bothell, Washington 98041, USA. psado@ora.fda.gov
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pubmed:publicationType |
Journal Article
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