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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1998-10-27
|
| pubmed:abstractText |
A total of 243 Escherichia coli strains isolated from patients with urinary tract infections (UTI) were investigated for the presence of pap, sfa and afa adhesin-encoding operons by using the polymerase chain reaction. It was found that 54%, 53% and 2% of the strains exhibited the pap, sfa and afa genotypes, respectively. Pap+ and/or sfa+ strains were more frequent in cases of acute pyelonephritis (94%) than in cases of cystitis (67%) (P < 0.001) and asymptomatic bacteriuria (57%) (P < 0.001). The pap and/or sfa operons were found in 90% of strains expressing mannose-resistant haemagglutination (MRHA) versus 37% of MRHA-negative strains (P < 0.001). The presence of pap and sfa operons was especially significant in strains belonging to MRHA types III (100%) (without P adhesins) and IVa (97%) (expressing the specific Gal-Gal binding typical of P adhesins). Both pap and sfa operons were closely associated with toxigenic E. coli producing alpha-haemolysin (Hly+) and/or the cytotoxic necrotizing factor type 1. There was an apparent correlation between the pap and sfa operons and the O serogroups of the strains. Thus, 93% of strains belonging to O1, O2, O4, O6, O7, O14, O15, O18, O22, O75 and O83 possessed pap and/or sfa operons, versus only 32% of strains belonging to other serogroups (P < 0.001). The results obtained in this study confirm the usefulness of our MRHA typing system for presumptive identification of pathogenic E. coli exhibiting different virulence factors. Thus, 85% of strains that possessed both pap and sfa adhesin-encoding operons showed MRHA types III or IVa previously associated with virulence of E. coli strains that cause UTI and bacteraemia.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adhesins, Escherichia coli,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hemolysin Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hlya protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/cytotoxic necrotizing factor type 1
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0923-2508
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
148
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
745-55
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9765858-Adhesins, Escherichia coli,
pubmed-meshheading:9765858-Adolescent,
pubmed-meshheading:9765858-Adult,
pubmed-meshheading:9765858-Aged,
pubmed-meshheading:9765858-Aged, 80 and over,
pubmed-meshheading:9765858-Bacterial Proteins,
pubmed-meshheading:9765858-Bacterial Toxins,
pubmed-meshheading:9765858-Bacteriuria,
pubmed-meshheading:9765858-Child,
pubmed-meshheading:9765858-Child, Preschool,
pubmed-meshheading:9765858-Cystitis,
pubmed-meshheading:9765858-Cytotoxins,
pubmed-meshheading:9765858-Escherichia coli,
pubmed-meshheading:9765858-Escherichia coli Infections,
pubmed-meshheading:9765858-Escherichia coli Proteins,
pubmed-meshheading:9765858-Female,
pubmed-meshheading:9765858-Fimbriae, Bacterial,
pubmed-meshheading:9765858-Genotype,
pubmed-meshheading:9765858-Hemolysin Proteins,
pubmed-meshheading:9765858-Humans,
pubmed-meshheading:9765858-Infant,
pubmed-meshheading:9765858-Infant, Newborn,
pubmed-meshheading:9765858-Male,
pubmed-meshheading:9765858-Middle Aged,
pubmed-meshheading:9765858-Operon,
pubmed-meshheading:9765858-Phenotype,
pubmed-meshheading:9765858-Polymerase Chain Reaction,
pubmed-meshheading:9765858-Pyelonephritis,
pubmed-meshheading:9765858-Urologic Diseases
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Detection of pap, sfa and afa adhesin-encoding operons in uropathogenic Escherichia coli strains: relationship with expression of adhesins and production of toxins.
|
| pubmed:affiliation |
Departamento de Microbiología y Parasitología, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, Spain.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|