Linked Life Data

9765408

Source:http://linkedlifedata.com/resource/pubmed/id/9765408

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1998-11-5
pubmed:abstractText
Dimerization of simian virus 40 T-antigen hexamers (TAgH) into double hexamers (TAgDH) on model DNA replication forks has been found to greatly stimulate T-antigen DNA helicase activity. To explore the interaction of TAgDH with DNA during unwinding, we examined the binding of TAgDH to synthetic DNA replication bubbles. Tests of replication bubble substrates containing different single-stranded DNA (ssDNA) lengths indicated that efficient formation of a TAgDH requires >/=40 nucleotides (nt) of ssDNA. DNase I probing of a substrate containing a 60-nt ssDNA bubble complexed with a TAgDH revealed that T antigen bound the substrate with twofold symmetry. The strongest protection was observed over the 5' junction on each strand, with 5 bp of duplex DNA and approximately 17 nt of adjacent ssDNA protected from nuclease cleavage. Stimulation of the T-antigen DNA helicase activity by an increase in ATP concentration caused the protection to extend in the 5' direction into the duplex region, while resulting in no significant changes to the 3' edge of strongest protection. Our data indicate that each TAgH encircles one ssDNA strand, with a different strand bound at each junction. The process of DNA unwinding results in each TAgH interacting with a greater length of DNA than was initially bound, suggesting the generation of a more highly processive helicase complex.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-1309914, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-1319087, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-1321135, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-1323237, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-1323692, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-1976634, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-2539565, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-2826443, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-2826446, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-2846276, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-3019672, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-7731998, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-7889572, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-7989299, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-8087839, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-8706121, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-9149137, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-9343236, http://linkedlifedata.com/resource/pubmed/commentcorrection/9765408-9369480
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8676-81
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Synthetic DNA replication bubbles bound and unwound with twofold symmetry by a simian virus 40 T-antigen double hexamer.
pubmed:affiliation
Department of Biochemistry and Kaplan Comprehensive Cancer Center, New York University Medical Center, New York, New York 10016, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't