| pubmed-article:9765225 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9765225 | lifeskim:mentions | umls-concept:C0005953 | lld:lifeskim |
| pubmed-article:9765225 | lifeskim:mentions | umls-concept:C0162597 | lld:lifeskim |
| pubmed-article:9765225 | lifeskim:mentions | umls-concept:C0538161 | lld:lifeskim |
| pubmed-article:9765225 | lifeskim:mentions | umls-concept:C0040690 | lld:lifeskim |
| pubmed-article:9765225 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:9765225 | pubmed:issue | 42 | lld:pubmed |
| pubmed-article:9765225 | pubmed:dateCreated | 1998-11-6 | lld:pubmed |
| pubmed-article:9765225 | pubmed:abstractText | Osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor (OCIF) is a recently identified cytokine that belongs to the tumor necrosis factor receptor superfamily and regulates bone mass by inhibiting osteoclastic bone resorption. The present study was undertaken to determine whether OPG/OCIF is produced in bone microenvironment and how the expression is regulated. A transcript for OPG/OCIF at 3.1 kilobases was detected in bone marrow stromal cells (ST2 and MC3T3-G2/PA6) as well as in osteoblastic cells (MC3T3-E1). Transforming growth factor-beta1 (TGF-beta1) markedly increased the steady-state level of OPG/OCIF mRNA in a dose-dependent manner, while TGF-beta1 suppressed the mRNA expression of tumor necrosis factor-related activation-induced cytokine (TRANCE)/receptor activator of NF-kappaB ligand (RANKL), a positive regulator of osteoclastogenesis to which OPG/OCIF binds. The effect of TGF-beta1 on the expression of OPG/OCIF mRNA was transient, with a peak level at 3-6 h. The up-regulation of OPG/OCIF mRNA by TGF-beta1 in ST2 cells did not require de novo protein synthesis and involved both a transcriptional and a post-transcriptional mechanism. Western blot analysis and an enzyme-linked immunosorbent assay revealed that TGF-beta1 significantly increased the secretion of OPG/OCIF protein by ST2 cells at 6-24 h. In murine bone marrow cultures, TGF-beta1 markedly inhibited the formation of tartrate-resistant acid phosphatase-positive multinucleated osteoclast-like cells in the presence of 1,25-dihydroxyvitamin D3, whose effect was significantly reversed by a neutralizing antibody against OPG/OCIF. These results suggest that TGF-beta1 negatively regulates osteoclastogenesis, at least in part, through the induction of OPG/OCIF by bone marrow stromal cells and that the balance between OPG/OCIF and TRANCE/RANKL in local environment may be an important determinant of osteoclastic bone resorption. | lld:pubmed |
| pubmed-article:9765225 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9765225 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9765225 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9765225 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9765225 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:9765225 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:9765225 | pubmed:author | pubmed-author:YamadaYY | lld:pubmed |
| pubmed-article:9765225 | pubmed:author | pubmed-author:WatanabeKK | lld:pubmed |
| pubmed-article:9765225 | pubmed:author | pubmed-author:IkedaKK | lld:pubmed |
| pubmed-article:9765225 | pubmed:author | pubmed-author:YangCC | lld:pubmed |
| pubmed-article:9765225 | pubmed:author | pubmed-author:TsudaEE | lld:pubmed |
| pubmed-article:9765225 | pubmed:author | pubmed-author:TakaiHH | lld:pubmed |
| pubmed-article:9765225 | pubmed:author | pubmed-author:KanematsuMM | lld:pubmed |
| pubmed-article:9765225 | pubmed:author | pubmed-author:HigashioKK | lld:pubmed |
| pubmed-article:9765225 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9765225 | pubmed:day | 16 | lld:pubmed |
| pubmed-article:9765225 | pubmed:volume | 273 | lld:pubmed |
| pubmed-article:9765225 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9765225 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9765225 | pubmed:pagination | 27091-6 | lld:pubmed |
| pubmed-article:9765225 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:9765225 | pubmed:meshHeading | pubmed-meshheading:9765225-... | lld:pubmed |
| pubmed-article:9765225 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9765225 | pubmed:articleTitle | Transforming growth factor-beta stimulates the production of osteoprotegerin/osteoclastogenesis inhibitory factor by bone marrow stromal cells. | lld:pubmed |
| pubmed-article:9765225 | pubmed:affiliation | Department of Geriatric Research, National Institute for Longevity Sciences, Obu, Aichi 474-8522, Japan. | lld:pubmed |
| pubmed-article:9765225 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9765225 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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