Linked Life Data

9764143

Source:http://linkedlifedata.com/resource/pubmed/id/9764143

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-10-19
pubmed:abstractText
A single application of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) to mouse skin results in an acute inflammatory response, with an influx of neutrophils and lymphocytes, epidermal hyperplasia and abnormal keratinocyte differentiation. This response is significantly inhibited by topical cyclosporin A (CyA). Although CyA is known to inhibit T-cell activation, the role of T cells in TPA-induced cutaneous inflammation is not well understood. In this study, we have used severe combined immunodeficient (SCID) mice, which carry a spontaneous mutation resulting in the absence of functional T and B lymphocytes, to examine whether lymphocytes are required for the TPA response in mouse skin and whether CyA inhibits the TPA response in SCID mice. A significant increase in epidermal and deep dermal inflammation was observed in both SCID and CB-17 mice 24 h after a single application of TPA (10 nmol) compared with vehicle (P < 0.05, n = 5-7). Simultaneous application of CyA (1.7 mumol) plus TPA resulted in a significant reduction in epidermal and deep inflammation at 24 h compared with TPA alone in SCID and CB-17 mice (P < 0.05, n = 7). In contrast to hairless mice, a variable increase in epidermal thickness was observed in both SCID and CB-17 mice after treatment with TPA at 24 and 72 h, which was not significantly affected by CyA. These data indicate that TPA-induced inflammation in mouse skin does not depend on lymphocytes. In addition, the inhibition of TPA-induced epidermal and deep dermal inflammation by CyA in SCID mouse skin suggests that CyA exerts effects on cutaneous inflammation in mice in the absence of functioning T cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0007-0963
pubmed:author
pubmed:issnType
Print
pubmed:volume
139
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
16-22
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Cyclosporin A inhibits 12-O-tetradecanoyl-phorbol-13-acetate-induced cutaneous inflammation in severe combined immunodeficient mice that lack functional lymphocytes.
pubmed:affiliation
Department of Dermatology, University of Michigan Medical School, Ann Arbor 48109-0609, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't