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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-11-5
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pubmed:abstractText |
The complete nucleotide sequence of pER371, a native plasmid in Streptococcus thermophilus ST137, was determined. A putative open reading frame coding for a replication protein, Rep371, was identified. A characteristic promoter sequence and ribosome-binding site were found upstream of rep371. Rep371 (247 amino acid residues) does not show homology with RepA and RepS of the small S. thermophilus cryptic plasmids pST1-No.29 and pST1 respectively. The plus-origin sequence and Rep371 are highly homologous to the corresponding elements of the Staphylococcus aureus plasmids pC194 and pSK89. A novel 140-nucleotide palindromic minusorigin sequence, which is structurally similar but does not show sequence homology to the palA region of pC194, was identified in pER371. A palindromic sequence capable of forming a putative hairpin structure was identified and subsequently recognized as being highly conserved among several lactococcal rolling-circle plasmids. Cloning vectors derived from pER371 should provide valuable gene-delivery vehicles for the genetic engineering of lactic acid bacteria.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/repS protein, Streptococcus...,
http://linkedlifedata.com/resource/pubmed/chemical/replication initiator protein
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0175-7598
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
174-80
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9763687-Amino Acid Sequence,
pubmed-meshheading:9763687-Bacterial Proteins,
pubmed-meshheading:9763687-Base Sequence,
pubmed-meshheading:9763687-DNA, Bacterial,
pubmed-meshheading:9763687-DNA Helicases,
pubmed-meshheading:9763687-DNA Replication,
pubmed-meshheading:9763687-DNA-Binding Proteins,
pubmed-meshheading:9763687-Genetic Vectors,
pubmed-meshheading:9763687-Molecular Sequence Data,
pubmed-meshheading:9763687-Nucleic Acid Conformation,
pubmed-meshheading:9763687-Open Reading Frames,
pubmed-meshheading:9763687-Plasmids,
pubmed-meshheading:9763687-Proteins,
pubmed-meshheading:9763687-Restriction Mapping,
pubmed-meshheading:9763687-Sequence Analysis, DNA,
pubmed-meshheading:9763687-Staphylococcus aureus,
pubmed-meshheading:9763687-Streptococcus,
pubmed-meshheading:9763687-Trans-Activators,
pubmed-meshheading:9763687-Viral Proteins
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pubmed:year |
1998
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pubmed:articleTitle |
Characterization of a novel Streptococcus thermophilus rolling-circle plasmid used for vector construction.
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pubmed:affiliation |
U.S. Department of Agriculture, ARS, Eastern Regional Research Center, Wyndmoor, PA 19038, USA. dsolaiman@arserrc.gov
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pubmed:publicationType |
Journal Article
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