Linked Life Data

9763144

Source:http://linkedlifedata.com/resource/pubmed/id/9763144

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-12-4
pubmed:abstractText
The cytokine, interleukin-6 (IL-6), is produced by osteoblasts and may in part mediate parathyroid hormone (PTH)-stimulated bone resorption. The goals of the present study were: (1) to examine PTH induction of IL-6 expression in 7-day-old mouse calvarial organ cultures; (2) to assess the role of intracellular signaling pathways in this model; and (3) to determine whether PTH regulates IL-6 expression by a transcriptional mechanism. Northern blot analysis of calvarial RNA showed that PTH(1-34) at 0.1-100 nmol/L induced IL-6 mRNA at 0.5 h with a peak at 2 h. Forskolin at 10 micromol/L and 8-bromocyclic-AMP at 3 mmol/L also induced IL-6 mRNA with a peak at 2 h. Phorbol myristate acetate induced IL-6 expression, whereas ionomycin and PTH(3-34) amide, an N-terminal-truncated PTH analog that has reduced ability to activate the cAMP-PKA pathway, were much less effective. PMA pretreatment of calvariae greatly blocked IL-6 mRNA induction by a subsequent dose of PMA and decreased induction by PTH and forskolin to a much lesser extent. A reverse-transcriptase polymerase chain reaction (RT-PCR) assay was used to measure IL-6 heterogeneous nuclear RNA (hnRNA) and mRNA. A 5' primer spanning exons 1 and 2 and a 3' primer complementary to exon 5 of the murine IL-6 gene were used to detect IL-6 mRNA as a 638 bp product. A 5' primer corresponding to intron 4 of the murine IL-6 gene and the 3' primer were used to detect IL-6 hnRNA as a 370 bp product. RT-PCR of total calvarial RNA showed that the induction of IL-6 hnRNA by PTH and other agonists was similar to their induction of IL-6 mRNA. These data support the conclusion that PTH transcriptionally induces IL-6 gene expression in murine calvarial organ cultures mainly through the cAMP-PKA signaling pathway.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
8756-3282
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
327-32
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9763144-8-Bromo Cyclic Adenosine Monophosphate, pubmed-meshheading:9763144-Animals, pubmed-meshheading:9763144-Animals, Newborn, pubmed-meshheading:9763144-Cell Nucleus, pubmed-meshheading:9763144-DNA Primers, pubmed-meshheading:9763144-Forskolin, pubmed-meshheading:9763144-Interleukin-6, pubmed-meshheading:9763144-Ionomycin, pubmed-meshheading:9763144-Mice, pubmed-meshheading:9763144-Organ Culture Techniques, pubmed-meshheading:9763144-Parathyroid Hormone, pubmed-meshheading:9763144-Parathyroid Hormone-Related Protein, pubmed-meshheading:9763144-Proteins, pubmed-meshheading:9763144-RNA, Heterogeneous Nuclear, pubmed-meshheading:9763144-RNA, Messenger, pubmed-meshheading:9763144-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:9763144-Signal Transduction, pubmed-meshheading:9763144-Skull, pubmed-meshheading:9763144-Tetradecanoylphorbol Acetate, pubmed-meshheading:9763144-Transcription, Genetic
pubmed:year
1998
pubmed:articleTitle
Parathyroid hormone induces interleukin-6 heterogeneous nuclear and messenger RNA expression in murine calvarial organ cultures.
pubmed:affiliation
Department of Oral Diagnosis, University of Connecticut Health Center, Farmington 06030, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.